Water-borne and plasma corticosterone are not correlated in spotted salamanders

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dc.contributor.authorMillikin, Alice R.en
dc.contributor.authorWoodley, Sarah K.en
dc.contributor.authorDavis, Drew R.en
dc.contributor.authorMoore, Ignacio T.en
dc.contributor.authorAnderson, James T.en
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2020-02-06T15:16:03Zen
dc.date.available2020-02-06T15:16:03Zen
dc.date.issued2019-11en
dc.description.abstractWater-borne hormone measurement is a noninvasive method suitable for amphibians of all sizes that are otherwise difficult to sample. For this method, containment-water is assayed for hormones released by the animal. Originally developed in fish, the method has expanded to amphibians, but requires additional species-specific validations. We wanted to determine physiological relevance of water-borne corticosterone in spotted salamanders (Ambystoma maculatum) by comparing concentrations to those taken using established corticosterone sampling methods, such as plasma. Using a mixture of field and laboratory studies, we compared water-borne corticosterone levels to other traditional methods of sampling corticosterone for spotted salamander larvae, metamorphs, and adults. Despite multiple attempts, and detecting differences between age groups, we found no correlations between water-borne and plasma corticosterone levels in any age group. Water-borne sampling measures a rate of release; whereas plasma is the concentration circulating in the blood. The unique units of measurement may inherently prevent correlations between the two. These two methods may also require different interpretations of the data and the physiological meaning. We also note caveats with the method, including how to account for differences in body size and life history stages. Collectively, our results illustrate the importance of careful validation of water-borne hormone levels in each species in order to understand its physiological significance.en
dc.description.notesU.S. Forest Service; Natural Resources Conservation Service; National Science Foundation, Grant/Award Number: 01A-1458952; West Virginia University Natural History Museum; National Institute of Food and Agriculture, Grant/Award Number: WVA00117; The Explorers Club Washington Group; Society of Wetland Scientists; Society of Wetland Scientists South Atlantic Chapter; West Virginia University Stitzel Graduate Enhancement Fund; Richard and Lois Bowman; National Institute of Health, Grant/Award Number: P51 OD011092en
dc.description.sponsorshipU.S. Forest ServiceUnited States Department of Agriculture (USDA)United States Forest Service; Natural Resources Conservation Service; National Science FoundationNational Science Foundation (NSF) [01A-1458952]; West Virginia University Natural History Museum; National Institute of Food and AgricultureUnited States Department of Agriculture (USDA)National Institute of Food and Agriculture [WVA00117]; Explorers Club Washington Group; Society of Wetland Scientists; Society of Wetland Scientists South Atlantic Chapter; National Institute of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [P51 OD011092]; West Virginia University Stitzel Graduate Enhancement Funden
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/ece3.5831en
dc.identifier.issn2045-7758en
dc.identifier.urihttp://hdl.handle.net/10919/96738en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAmbystoma maculatumen
dc.subjectglucocorticoiden
dc.subjecthormonesen
dc.subjectnoninvasiveen
dc.subjectvalidationen
dc.titleWater-borne and plasma corticosterone are not correlated in spotted salamandersen
dc.title.serialEcology and Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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