Label-free analysis of physiological hyaluronan size distribution with a solid-state nanopore sensor

Simple item page
dc.contributor.authorRivas, Felipeen
dc.contributor.authorZahid, Osama K.en
dc.contributor.authorReesink, Heidi L.en
dc.contributor.authorPeal, Bridgette T.en
dc.contributor.authorNixon, Alan J.en
dc.contributor.authorDeAngelis, Paul L.en
dc.contributor.authorSkardal, Aleksanderen
dc.contributor.authorRahbar, Elahehen
dc.contributor.authorHall, Adam R.en
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2019-02-07T14:44:22Zen
dc.date.available2019-02-07T14:44:22Zen
dc.date.issued2018-03-12en
dc.description.abstractHyaluronan (or hyaluronic acid, HA) is a ubiquitous molecule that plays critical roles in numerous physiological functions in vivo, including tissue hydration, inflammation, and joint lubrication. Both the abundance and size distribution of HA in biological fluids are recognized as robust indicators of various pathologies and disease progressions. However, such analyses remain challenging because conventional methods are not sufficiently sensitive, have limited dynamic range, and/or are only semi-quantitative. Here we demonstrate label-free detection and molecular weight discrimination of HA with a solid-state nanopore sensor. We first employ synthetic HA polymers to validate the measurement approach and then use the platform to determine the size distribution of as little as 10 ng of HA extracted directly from synovial fluid in an equine model of osteoarthritis. Our results establish a quantitative method for assessment of a significant molecular biomarker that bridges a gap in the current state of the art.en
dc.description.notesThe authors wish to acknowledge Courtney Smith for early contributions to the measurements and Glenn Prestwich for helpful discussions. This work was supported by start-up funds to E.R. and A.R.H. from Wake Forest University Health Sciences.en
dc.description.sponsorshipWake Forest University Health Sciencesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41467-018-03439-xen
dc.identifier.issn2041-1723en
dc.identifier.other1037en
dc.identifier.pmid29531292en
dc.identifier.urihttp://hdl.handle.net/10919/87523en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleLabel-free analysis of physiological hyaluronan size distribution with a solid-state nanopore sensoren
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41467-018-03439-x.pdf
Size:
1.92 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, School of Biomedical Engineering and Sciences