Immunoregulatory mechanisms underlying prevention of colitis-associated colorectal cancer by probiotic bacteria

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dc.contributorVirginia Techen
dc.contributor.authorBassaganya-Riera, Josepen
dc.contributor.authorViladomiu, Monicaen
dc.contributor.authorPedragosa, Mireiaen
dc.contributor.authorDe Simone, Claudioen
dc.contributor.authorHontecillas, Raquelen
dc.date.accessed2014-04-30en
dc.date.accessioned2014-06-17T20:12:07Zen
dc.date.available2014-06-17T20:12:07Zen
dc.date.issued2012-04-12en
dc.description.abstractBackground: Inflammatory bowel disease (IBD) increases the risk of colorectal cancer. Probiotic bacteria produce immunoregulatory metabolites in vitro such as conjugated linoleic acid (CLA), a polyunsaturated fatty acid with potent anti-carcinogenic effects. This study aimed to investigate the cellular and molecular mechanisms underlying the efficacy of probiotic bacteria in mouse models of cancer. Methodology/Principal Findings: The immune modulatory mechanisms of VSL#3 probiotic bacteria and CLA were investigated in mouse models of inflammation-driven colorectal cancer. Colonic specimens were collected for histopathology, gene expression and flow cytometry analyses. Immune cell subsets in the mesenteric lymph nodes (MLN), spleen and colonic lamina propria lymphocytes (LPL) were phenotypically and functionally characterized. Mice treated with CLA or VSL#3 recovered faster from the acute inflammatory phase of disease and had lower disease severity in the chronic, tumor-bearing phase of disease. Adenoma and adenocarcinoma formation was also diminished by both treatments. VSL#3 increased the mRNA expression of TNF-α, angiostatin and PPAR γ whereas CLA decreased COX-2 levels. Moreover, VSL#3-treated mice had increased IL-17 expression in MLN CD4+ T cells and accumulation of Treg LPL and memory CD4+ T cells. Conclusions/Significance: Both CLA and VSL#3 suppressed colon carcinogenesis, although VSL#3 showed greater anti-carcinogenic and anti-inflammatory activities than CLA. Mechanistically, CLA modulated expression of COX-2 levels in the colonic mucosa, whereas VSL#3 targeted regulatory mucosal CD4+ T cell responses.en
dc.identifier.citationBassaganya-Riera J, Viladomiu M, Pedragosa M, De Simone C, Hontecillas R (2012) Immunoregulatory Mechanisms Underlying Prevention of Colitis-Associated Colorectal Cancer by Probiotic Bacteria. PLoS ONE 7(4): e34676. doi:10.1371/journal.pone.0034676en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0034676en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/48992en
dc.identifier.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0034676en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCancer treatmenten
dc.subjectColonen
dc.subjectColorectal canceren
dc.subjectInflammationen
dc.subjectInflammatory diseaseen
dc.subjectLinoleic aciden
dc.subjectProbioticsen
dc.subjectT cellsen
dc.titleImmunoregulatory mechanisms underlying prevention of colitis-associated colorectal cancer by probiotic bacteriaen
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden

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