Soybean Salt Tolerance 1 (GmST1) Reduces ROS Production, Enhances ABA Sensitivity, and Abiotic Stress Tolerance in Arabidopsis thaliana

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dc.contributor.authorRen, Shuxinen
dc.contributor.authorLyle, Chimeraen
dc.contributor.authorJiang, Guo-liangen
dc.contributor.authorPenumala, Abhisheken
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2019-04-30T12:45:22Zen
dc.date.available2019-04-30T12:45:22Zen
dc.date.issued2016-04-11en
dc.description.abstractAbiotic stresses, including high soil salinity, significantly reduce crop production worldwide. Salt tolerance in plants is a complex trait and is regulated by multiple mechanisms. Understanding the mechanisms and dissecting the components on their regulatory pathways will provide new insights, leading to novel strategies for the improvement of salt tolerance in agricultural and economic crops of importance. Here we report that soybean salt tolerance 1, named GmST1, exhibited strong tolerance to salt stress in the Arabidopsis transgenic lines. The GmST1-overexpressed Arabidopsis also increased sensitivity to ABA and decreased production of reactive oxygen species under salt stress. In addition, GmST1 significantly improved drought tolerance in Arabidopsis transgenic lines. GmST1 belongs to a 3-prime part of Glyma.03g171600 gene in the current version of soybean genome sequence annotation. However, comparative reverse transcription-polymerase chain reaction analysis around Glyma.03g171600 genomic region confirmed that GmST1 might serve as an intact gene in soybean leaf tissues. Unlike Glyma.03g171600 which was not expressed in leaves, GmST1 was strongly induced by salt treatment in the leaf tissues. By promoter analysis, a TATA box was detected to be positioned close to GmST1 start codon and a putative ABRE and a DRE cis-acting elements were identified at about 1 kb upstream of GmST1 gene. The data also indicated that GmST1-transgenic lines survived under drought stress and showed a significantly lower water loss than non-transgenic lines. In summary, our results suggest that overexpression of GmST1 significantly improves Arabidopsis tolerance to both salt and drought stresses and the gene may be a potential candidate for genetic engineering of salt- and drought-tolerant crops.en
dc.description.notesWe would like to thank USDA for its Evans Allen formula funds to support the research programs at the Virginia State University (to SR and G-LJ). Dr. Sarah Weeda, who passed away in a car accident in summer of 2015, contributed significantly in conduction of the designed experiments. We would also like to thank Ms. Roz Stein for her editing and proof reading of the manuscript. The authors would also like to thank reviewers for their valuable comments. This article is a contribution of the Virginia State University, Agricultural Research Station (Journal series No. 329).en
dc.description.sponsorshipUSDAen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2016.00445en
dc.identifier.eissn1664-462Xen
dc.identifier.other445en
dc.identifier.pmid27148284en
dc.identifier.urihttp://hdl.handle.net/10919/89253en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsoybeanen
dc.subjectGmST1 overexpressionen
dc.subjectsalt toleranceen
dc.subjectdrought toleranceen
dc.subjectROS productionen
dc.subjectABA sensitivityen
dc.titleSoybean Salt Tolerance 1 (GmST1) Reduces ROS Production, Enhances ABA Sensitivity, and Abiotic Stress Tolerance in Arabidopsis thalianaen
dc.title.serialFrontiers In Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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