Tollip Deficiency Alters Atherosclerosis and Steatosis by Disrupting Lipophagy

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dc.contributor.authorChen, Keqiangen
dc.contributor.authorYuan, Ruoxien
dc.contributor.authorZhang, Yaoen
dc.contributor.authorGeng, Shuoen
dc.contributor.authorLi, Liwuen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2018-10-15T14:20:47Zen
dc.date.available2018-10-15T14:20:47Zen
dc.date.issued2017-04-10en
dc.description.abstractBackground Compromised lipophagy with unknown mechanisms may be critically involved in the intracellular accumulation of lipids, contributing to elevated atherosclerosis and liver steatosis. We hypothesize that toll‐interacting protein (Tollip), a key innate immune molecule involved in the fusion of autolysosome, may play a significant role in lipophagy and modulate lipid accumulation during the pathogenesis of atherosclerosis and liver steatosis. Methods and Results By comparing mice fed with either a Western high‐fat diet or a regular chow diet, we observed that both atherosclerosis and liver steatosis were aggravated in apolipoprotein E–deficient (ApoE−/−)/Tollip−/− mice as compared with ApoE−/− mice. Through electron microscopy analyses, we observed compromised fusion of lipid droplets with lysosomes within aortic macrophages as well as liver hepatocytes from ApoE−/−/Tollip−/− mice as compared with ApoE−/− mice. As a molecular indicator for disrupted lysosome fusion, the levels of p62 were significantly elevated in aortic and liver tissues from ApoE−/−/Tollip−/− mice. Molecules involved in facilitating lipophagy completion such as Ras‐related protein 7 and gamma‐aminobutyric acid receptor‐associated protein were reduced in ApoE−/−/Tollip−/− mice as compared with ApoE−/− mice. Intriguingly, ApoE−/−/Tollip−/− mice had reduced circulating levels of inflammatory cytokines such as tumor necrosis factor‐α and increased levels of transforming growth factor‐β. The reduced inflammation due to Tollip deficiency is consistent with a stable atherosclerotic plaque phenotype with increased levels of plaque collagen and smooth muscle cells in ApoE−/−/Tollip−/− mice. Conclusions Tollip deficiency selectively leads to enlarged yet stable atherosclerotic plaques, increased circulating lipids, liver steatosis, and reduced inflammation. Compromised lipophagy and reduced expression of inflammatory mediators due to Tollip deficiency may be the underlying causes. Our data suggest that lipid accumulation and inflammation may be intertwined yet independent processes during the progression of atherosclerosis and steatosis.en
dc.description.versionPublisheden
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1161/JAHA.116.004078en
dc.identifier.issn2047-9980en
dc.identifier.urihttp://hdl.handle.net/10919/85372en
dc.publisherWolters Kluwer Healthen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleTollip Deficiency Alters Atherosclerosis and Steatosis by Disrupting Lipophagyen
dc.title.serialJournal of the American Heart Associationen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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