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The assembly of integrated rat intestinal-hepatocyte cultures

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dc.contributor.authorKothari, Anjaneyen
dc.contributor.authorRajagopalan, Padmavathyen
dc.contributor.departmentChemical Engineeringen
dc.contributor.departmentInstitute for Critical Technology and Applied Science (ICTAS)en
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2020-02-07T14:34:38Zen
dc.date.available2020-02-07T14:34:38Zen
dc.date.issued2019-11en
dc.description.abstractThe jejunum is the segment of the small intestine responsible for several metabolism and biotransformation functions. In this report, we have cultured rat jejunum explants in vitro and integrated them with hepatocyte cultures. We have also investigated the changes in jejunum function at different locations since spatial variations in intestinal functions have been reported previously. We divided the length of the rat jejunum into three distinct regions of approximately 9 cm each. We defined the regions as proximal (adjacent to the duodenum), medial, and distal (adjacent to the ileum). Spatiotemporal variations in functions were observed between these regions within the jejunum. Alkaline phosphatase activity (a marker of enterocyte function), decreased twofold between the proximal and distal regions at 4 hr. Lysozyme activity (a marker of Paneth cell function) increased from the proximal to the distal jejunum by 40% at 24 hr. Mucin-covered areas, a marker of goblet cell function, increased by twofold between the proximal and distal segments of the jejunum at 24 hr. When hepatocytes were integrated with proximal jejunum explants, statistically higher urea (similar to 2.4-fold) and mucin (57%) production were observed in the jejunum explants. The integrated intestine-liver cultures can be used as a platform for future investigations.en
dc.description.notesNational Science Foundation USA, Grant/Award Number: NSF:CBET-1510920en
dc.description.sponsorshipNational Science Foundation USANational Science Foundation (NSF) [NSF:CBET-1510920]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/btm2.10146en
dc.identifier.eissn2380-6761en
dc.identifier.otherUNSP e10146en
dc.identifier.urihttp://hdl.handle.net/10919/96761en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectintegrated culturesen
dc.subjectliveren
dc.subjectsmall intestineen
dc.titleThe assembly of integrated rat intestinal-hepatocyte culturesen
dc.title.serialBioengineering & Translational Medicineen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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