Characterization of sequentially-staged cancer cells using electrorotation

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dc.contributor.authorTrainito, Claudia I.en
dc.contributor.authorSweeney, Daniel C.en
dc.contributor.authorČemazăr, Jakaen
dc.contributor.authorSchmelz, Eva M.en
dc.contributor.authorFrançaisen
dc.contributor.authorLe Pioufle, Brunoen
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2019-11-13T13:49:22Zen
dc.date.available2019-11-13T13:49:22Zen
dc.date.issued2019-09-19en
dc.description.abstractThe identification and separation of cells from heterogeneous populations is critical to the diagnosis of diseases. Label-free methodologies in particular have been developed to manipulate individual cells using properties such as density and morphology. The electrical properties of malignant cells, including the membrane capacitance and cytoplasmic conductivity, have been demonstrated to be altered compared to non-malignant cells of similar origin. Here, we exploit these changes to characterize individual cells in a sequentially-staged in vitro cancer model using electrorotation (EROT)—the rotation of a cell induced by a rotating electric field. Using a microfabricated device, a dielectrophoretic force to suspend cells while measuring their angular velocity resulting from an EROT force applied at frequencies between 3 kHz to 10 MHz. We experimentally determine the EROT response for cells at three stages of malignancy and analyze the resultant spectra by considering models that include the effect of the cell membrane alone (single-shell model) and the combined effect of the cell membrane and nucleus (double-shell model). We find that the cell membrane is largely responsible for a given cell’s EROT response between 3 kHz and 10 MHz. Our results also indicate that membrane capacitance, membrane conductance, and cytoplasmic conductivity increase with an increasingly malignant phenotype. Our results demonstrate the potential of using electrorotation as a means making of non-invasive measurements to characterize the dielectric properties of cancer cells.en
dc.description.sponsorshipThe authors would like to acknowledge the generous financial support provided by the LabeX LaSIPS (ANR-10-LABX-0040-LaSIPS) managed by the French National Research Agency under the “Investissements d’avenir” program (nANR-11-IDEX-0003-02) and COST TD1104, www.electroporation.net. The authors would like to thank the NSF CAREER Award CBET-1055913, the NSF IGERT DGE-09661, and the NIH R01- CA213423 for their gracious support of this work. The authors would also like to acknowledge project support from ENS Paris Saclay and Virginia Tech.en
dc.format.extent18 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationTrainito CI, Sweeney DC, Čemazăr J, Schmelz EM, Français O, Le Pioufle B, et al. (2019) Characterization of sequentially-staged cancer cells using electrorotation. PLoS ONE 14(9): e0222289. https://doi.org/10.1371/journal.pone.0222289en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0222289en
dc.identifier.issue9en
dc.identifier.urihttp://hdl.handle.net/10919/95515en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleCharacterization of sequentially-staged cancer cells using electrorotationen
dc.title.serialPLOS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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