Plasmalogen Biosynthesis by Anaerobic Bacteria: Identification of a Two-Gene Operon Responsible for Plasmalogen Production in Clostridium perfringens

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dc.contributor.authorJackson, David R.en
dc.contributor.authorCassilly, Chelsi D.en
dc.contributor.authorPlichta, Damian R.en
dc.contributor.authorVlamakis, Heraen
dc.contributor.authorLiu, Hualanen
dc.contributor.authorMelville, Stephen B.en
dc.contributor.authorXavier, Ramnik J.en
dc.contributor.authorClardy, Jonen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2021-06-02T12:55:20Zen
dc.date.available2021-06-02T12:55:20Zen
dc.date.issued2021-01-15en
dc.description.abstractPlasmalogens are vinyl ether-containing lipids produced by mammals and bacteria. The aerobic biosynthetic pathway in eukaryotes and bacteria is known, but the anaerobic pathway has remained a mystery. Here, we describe a two-gene operon (plasmalogen synthase, pis) responsible for plasmalogen production in the anaerobic bacterium Clostridium perfringens. While aerobic plasmalogen biosynthesis involves an oxidative conversion of an ether to a vinyl ether, anaerobic plasmalogen biosynthesis uses the reductive conversion of an ester to an aldehyde equivalent. Heterologous expression of the C. perfringens pis operon in E. coli conferred the ability to produce plasmalogens. The pls operon is predicted to encode a multidomain complex similar to benzoyl-CoA reductase/hydroxylacyl-CoA dehydratase (BCR/HAD) enzymes. Versions of this operon can be found in a wide range of obligate and facultative anaerobic bacteria, including many human gut microbes.en
dc.description.notesWe thank H. Goldfine (University of Pennsylvania) for kindly providing insights from his laboratory's work on plasmalogens in anaerobic bacteria, and for inspiring and supporting us early on in our research efforts. We acknowledge funding support from the National Institutes of Health (Grant Nos. R01AT009708 (J.C. and R.J.X.), F32GM122233 (D.R.J.), and F32AT010415 (C.D.C.).en
dc.description.sponsorshipNational Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01AT009708, F32GM122233, F32AT010415]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1021/acschembio.0c00673en
dc.identifier.eissn1554-8937en
dc.identifier.issn1554-8929en
dc.identifier.issue1en
dc.identifier.pmid33350306en
dc.identifier.urihttp://hdl.handle.net/10919/103561en
dc.identifier.volume16en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titlePlasmalogen Biosynthesis by Anaerobic Bacteria: Identification of a Two-Gene Operon Responsible for Plasmalogen Production in Clostridium perfringensen
dc.title.serialACS Chemical Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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