3D electron microscopy and volume-based bouton sorting reveal the selectivity of inputs onto geniculate relay cell and interneuron dendrite segments

dc.contributor.authorMaher, Erin E.en
dc.contributor.authorBriegel, Alex C.en
dc.contributor.authorImtiaz, Shahroziaen
dc.contributor.authorFox, Michael A.en
dc.contributor.authorGolino, Hudsonen
dc.contributor.authorErisir, Aleven
dc.date.accessioned2023-10-10T13:27:15Zen
dc.date.available2023-10-10T13:27:15Zen
dc.date.issued2023-03en
dc.description.abstractIntroductionThe visual signals evoked at the retinal ganglion cells are modified and modulated by various synaptic inputs that impinge on lateral geniculate nucleus cells before they are sent to the cortex. The selectivity of geniculate inputs for clustering or forming microcircuits on discrete dendritic segments of geniculate cell types may provide the structural basis for network properties of the geniculate circuitry and differential signal processing through the parallel pathways of vision. In our study, we aimed to reveal the patterns of input selectivity on morphologically discernable relay cell types and interneurons in the mouse lateral geniculate nucleus. MethodsWe used two sets of Scanning Blockface Electron Microscopy (SBEM) image stacks and Reconstruct software to manually reconstruct of terminal boutons and dendrite segments. First, using an unbiased terminal sampling (UTS) approach and statistical modeling, we identified the criteria for volume-based sorting of geniculate boutons into their putative origins. Geniculate terminal boutons that were sorted in retinal and non-retinal categories based on previously described mitochondrial morphology, could further be sorted into multiple subpopulations based on their bouton volume distributions. Terminals deemed non-retinal based on the morphological criteria consisted of five distinct subpopulations, including small-sized putative corticothalamic and cholinergic boutons, two medium-sized putative GABAergic inputs, and a large-sized bouton type that contains dark mitochondria. Retinal terminals also consisted of four distinct subpopulations. The cutoff criteria for these subpopulations were then applied to datasets of terminals that synapse on reconstructed dendrite segments of relay cells or interneurons. ResultsUsing a network analysis approach, we found an almost complete segregation of retinal and cortical terminals on putative X-type cell dendrite segments characterized by grape-like appendages and triads. On these cells, interneuron appendages intermingle with retinal and other medium size terminals to form triads within glomeruli. In contrast, a second, presumed Y-type cell displayed dendrodendritic puncta adherentia and received all terminal types without a selectivity for synapse location; these were not engaged in triads. Furthermore, the contribution of retinal and cortical synapses received by X-, Y- and interneuron dendrites differed such that over 60% of inputs to interneuron dendrites were from the retina, as opposed to 20% and 7% to X- and Y-type cells, respectively. ConclusionThe results underlie differences in network properties of synaptic inputs from distinct origins on geniculate cell types.en
dc.description.notesThis work was supported by U01 NS122040 (AE) and UVA Graduate School of Arts and Sciences Research Funds (AE).en
dc.description.sponsorshipUVA Graduate School of Arts and Sciences Research Funds; [U01 NS122040]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fnana.2023.1150747en
dc.identifier.issn1662-5129en
dc.identifier.other1150747en
dc.identifier.pmid37007643en
dc.identifier.urihttp://hdl.handle.net/10919/116438en
dc.identifier.volume17en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSBEMen
dc.subjectLGNen
dc.subjectvisual thalamusen
dc.subjectunbiased samplingen
dc.subjectretinogeniculateen
dc.subjectcorticogeniculateen
dc.title3D electron microscopy and volume-based bouton sorting reveal the selectivity of inputs onto geniculate relay cell and interneuron dendrite segmentsen
dc.title.serialFrontiers in Neuroanatomyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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