A Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markers

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dc.contributor.authorPark, Kyung Sooen
dc.contributor.authorShin, Seung Wonen
dc.contributor.authorJang, Min Suen
dc.contributor.authorShin, Woojungen
dc.contributor.authorYang, Kisuken
dc.contributor.authorMin, Junhongen
dc.contributor.authorCho, Seung-Wooen
dc.contributor.authorOh, Byung-Keunen
dc.contributor.authorBae, Jong Wooken
dc.contributor.authorJung, Sunghwanen
dc.contributor.authorChoi, Jeong-Wooen
dc.contributor.authorUm, Soong Hoen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessioned2019-01-24T15:40:36Zen
dc.date.available2019-01-24T15:40:36Zen
dc.date.issued2015-12-18en
dc.description.abstractAccurate cancer diagnosis often requires extraction and purification of genetic materials from cells, and sophisticated instrumentations that follow. Otherwise in order to directly treat the diagnostic materials to cells, multiple steps to optimize dose concentration and treatment time are necessary due to diversity in cellular behaviors. These processes may offer high precision but hinder fast analysis of cancer, especially in clinical situations that need rapid detection and characterization of cancer. Here we present a novel fluorescent tile DNA nanostructure delivered to cancer cytosol by employing nanoparticle technology. Its structural anisotropicity offers easy manipulation for multifunctionalities, enabling the novel DNA nanostructure to detect intracellular cancer RNA markers with high specificity within 30 minutes post treatment, while the nanoparticle property bypasses the requirement of treatment optimization, effectively reducing the complexity of applying the system for cancer diagnosis. Altogether, the system offers a precise and rapid detection of cancer, suggesting the future use in the clinical fields.en
dc.description.notesWe especially thank Mr. Woo Chul Song for his ardent help and discussion. This work was supported by grants from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant no. HI14C3301) and by Basic Science Research Programs through the National Research Foundation (NRF) funded by the Ministry of Science ICT and Future Planning (Grant Nos. 2013R1A1A1058670 and 2013R1A1A2016781) and by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP) (2013K1A4A3055268).en
dc.description.sponsorshipKorea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI14C3301]; Basic Science Research Programs through the National Research Foundation (NRF) - Ministry of Science ICT and Future Planning [2013R1A1A1058670, 2013R1A1A2016781]; Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning (MSIP) [2013K1A4A3055268]en
dc.format.extent8en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep18497en
dc.identifier.issn2045-2322en
dc.identifier.other18497en
dc.identifier.pmid26678430en
dc.identifier.urihttp://hdl.handle.net/10919/86876en
dc.identifier.volume5en
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecttargeted drug-deliveryen
dc.subjectliving cellsen
dc.subjecthybridizationen
dc.subjecttherapyen
dc.subjectprobesen
dc.titleA Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markersen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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