An Intertwined Evolutionary History of Methanogenic Archaea and Sulfate Reduction

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dc.contributorVirginia Techen
dc.contributor.authorSusanti, Dwien
dc.contributor.authorMukhopadhyay, Biswarupen
dc.date.accessed2014-05-07en
dc.date.accessioned2014-06-17T20:12:08Zen
dc.date.available2014-06-17T20:12:08Zen
dc.date.issued2012-09-21en
dc.description.abstractHydrogenotrophic methanogenesis and dissimilatory sulfate reduction, two of the oldest energy conserving respiratory systems on Earth, apparently could not have evolved in the same host, as sulfite, an intermediate of sulfate reduction, inhibits methanogenesis. However, certain methanogenic archaea metabolize sulfite employing a deazaflavin cofactor (F420)-dependent sulfite reductase (Fsr) where N- and C-terminal halves (Fsr-N and Fsr-C) are homologs of F420H2 dehydrogenase and dissimilatory sulfite reductase (Dsr), respectively. From genome analysis we found that Fsr was likely assembled from freestanding Fsr-N homologs and Dsr-like proteins (Dsr-LP), both being abundant in methanogens. Dsr-LPs fell into two groups defined by following sequence features: Group I (simplest), carrying a coupled siroheme-[Fe4-S4] cluster and sulfite-binding Arg/Lys residues; Group III (most complex), with group I features, a Dsr-type peripheral [Fe4-S4] cluster and an additional [Fe4-S4] cluster. Group II Dsr-LPs with group I features and a Dsr-type peripheral [Fe4-S4] cluster were proposed as evolutionary intermediates. Group III is the precursor of Fsr-C. The freestanding Fsr-N homologs serve as F420H2 dehydrogenase unit of a putative novel glutamate synthase, previously described membrane-bound electron transport system in methanogens and of assimilatory type sulfite reductases in certain haloarchaea. Among archaea, only methanogens carried Dsr-LPs. They also possessed homologs of sulfate activation and reduction enzymes. This suggested a shared evolutionary history for methanogenesis and sulfate reduction, and Dsr-LPs could have been the source of the oldest (3.47-Gyr ago) biologically produced sulfide deposit.en
dc.description.sponsorshipThis project has been supported by NASA Astrobiology: Exobiology and Evolutionary Biology grants NNG05GP24G and NNX09AV28G to BM and the National Science Foundation grant MCB1020458 to BM and Bob B. Buchanan. DS received a graduate fellowship from the Virginia Tech Genetics, Bioinformatics and Computational Biology Ph.D. program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.citationSusanti D, Mukhopadhyay B. An intertwined evolutionary history of methanogenic archaea and sulfate reduction. PLoS One. 2012;7:e45313.  http://www.ncbi.nlm.nih.gov/pubmed/23028926en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0045313en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/48996en
dc.identifier.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0045313en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectArchaean biologyen
dc.subjectGenome evolutionen
dc.subjectHydrothermal ventsen
dc.subjectMethanogensen
dc.subjectPhylogenetic analysisen
dc.subjectSulfatesen
dc.subjectSulfidesen
dc.subjectSulfitesen
dc.titleAn Intertwined Evolutionary History of Methanogenic Archaea and Sulfate Reductionen
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden

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