Tissue-Associated Bacterial Alterations in Rectal Carcinoma Patients Revealed by 16S rRNA Community Profiling

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dc.contributor.authorThomas, Andrew M.en
dc.contributor.authorJesus, Eliane C.en
dc.contributor.authorLopes, Ademaren
dc.contributor.authorAguiar, Samuel, Jr.en
dc.contributor.authorBegnami, Maria D.en
dc.contributor.authorRocha, Rafael M.en
dc.contributor.authorCarpinetti, Paola Avelaren
dc.contributor.authorCamargo, Anamaria A.en
dc.contributor.authorHoffmann, Christianen
dc.contributor.authorFreitas, Helano C.en
dc.contributor.authorSilva, Israel T.en
dc.contributor.authorNunes, Diana N.en
dc.contributor.authorSetubal, Joao C.en
dc.contributor.authorDias-Neto, Emmanuelen
dc.date.accessioned2019-11-01T19:24:39Zen
dc.date.available2019-11-01T19:24:39Zen
dc.date.issued2016-12-09en
dc.description.abstractSporadic and inflammatory forms of colorectal cancer (CRC) account for more than 80% of cases. Recent publications have shown mechanistic evidence for the involvement of gut bacteria in the development of both CRC-forms. Whereas, colon and rectal cancer have been routinely studied together as CRC, increasing evidence show these to be distinct diseases. Also, the common use of fecal samples to study microbial communities may reflect disease state but possibly not the tumor microenvironment. We performed this study to evaluate differences in bacterial communities found in tissue samples of 18 rectal-cancer subjects when compared to 18 non-cancer controls. Samples were collected during exploratory colonoscopy (non-cancer group) or during surgery for tumor excision (rectal-cancer group). High throughput 16S rRNA amplicon sequencing of the V4V5 region was conducted on the Ion PGM platform, reads were filtered using Qiime and clustered using UPARSE. We observed significant increases in species richness and diversity in rectal cancer samples, evidenced by the total number of OTUs and the Shannon and Simpson indexes. Enterotyping analysis divided our cohort into two groups, with the majority of rectal cancer samples clustering into one enterotype, characterized by a greater abundance of Bacteroides and Dorea. At the phylum level, rectal-cancer samples had increased abundance of candidate phylum OD1 (also known as Parcubacteria) whilst non-cancer samples had increased abundance of Planctomycetes. At the genera level, rectal-cancer samples had higher abundances of Bacteroides, Phascolarctobacterium, Parabacteroides, Desulfovibrio, and Odoribacter whereas non-cancer samples had higher abundances of Pseudomonas, Escherichia, Acinetobacter, Lactobacillus, and Bacillus. Two Bacteroides fragilis OTUs were more abundant among rectal-cancer patients seen through 16S rRNA amplicon sequencing, whose presence was confirmed by immunohistochemistry and enrichment verified by digital droplet PCR. Our findings point to increased bacterial richness and diversity in rectal cancer, along with several differences in microbial community composition. Our work is the first to present evidence for a possible role of bacteria such as B. fragilis and the phylum Parcubacteria in rectal cancer, emphasizing the need to study tissue-associated bacteria and specific regions of the gastrointestinal tract in order to better understand the possible links between the microbiota and rectal cancer.en
dc.description.notesAT was supported by a fellowship from FAPESP (2015/01507-7). CH was supported by CAPES grant (88887.062078/2014-00) and FAPESP grant (2013/07914-8). This project was supported by PRONON (SIPAR 25000.055.167/2015-23), by Associacao Beneficiente Alzira Denise Hertzog Silva (ABADHS) and by CAPES grant 3385/2013.en
dc.description.sponsorshipFAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/01507-7, 2013/07914-8]; CAPESCAPES [88887.062078/2014-00, 3385/2013]; PRONON [25000.055.167/2015-23]; Associacao Beneficiente Alzira Denise Hertzog Silva (ABADHS)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fcimb.2016.00179en
dc.identifier.issn2235-2988en
dc.identifier.other179en
dc.identifier.pmid28018861en
dc.identifier.urihttp://hdl.handle.net/10919/95230en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmucosa-associated microbiotaen
dc.subjectrectal canceren
dc.subject16S rRNA gene sequencingen
dc.subjectBacteroides fragilisen
dc.subjectBacterial diversity and community compositionen
dc.titleTissue-Associated Bacterial Alterations in Rectal Carcinoma Patients Revealed by 16S rRNA Community Profilingen
dc.title.serialFrontiers in Cellular and Infection Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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