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Immunomagnetic separation of tumor initiating cells by screening two surface markers

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dc.contributor.authorSun, Chenen
dc.contributor.authorHsieh, Yuan-Pangen
dc.contributor.authorMa, Saien
dc.contributor.authorGeng, Shuoen
dc.contributor.authorCao, Zhenningen
dc.contributor.authorLi, Liwuen
dc.contributor.authorLu, Changen
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentChemical Engineeringen
dc.date.accessioned2019-01-11T15:22:10Zen
dc.date.available2019-01-11T15:22:10Zen
dc.date.issued2017-01-11en
dc.description.abstractIsolating tumor initiating cells (TICs) often requires screening of multiple surface markers, sometimes with opposite preferences. This creates a challenge for using bead-based immunomagnetic separation (IMS) that typically enriches cells based on one abundant marker. Here, we propose a new strategy that allows isolation of CD44(+)/CD24(-) TICs by IMS involving both magnetic beads coated by anti-CD44 antibody and nonmagnetic beads coated by anti-CD24 antibody (referred to as two-bead IMS). Cells enriched with our approach showed significant enhancement in TIC marker expression (examined by flow cytometry) and improved tumorsphere formation efficiency. Our method will extend the application of IMS to cell subsets characterized by multiple markers.en
dc.description.notesThis work was supported by NIH grants CA174577, EB017235, and EB019123. The authors would like to thank Dr. Albert Baldwin at University of North Carolina for generously providing SUM 149 cell line to us as a gift.en
dc.description.sponsorshipNIH [CA174577, EB017235, EB019123]en
dc.format.extent8en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep40632en
dc.identifier.issn2045-2322en
dc.identifier.other40632en
dc.identifier.pmid28074882en
dc.identifier.urihttp://hdl.handle.net/10919/86670en
dc.identifier.volume7en
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcancer stem-cellsen
dc.subjectin-vitro propagationen
dc.subjecttherapeutic implicationsen
dc.subjectprospective identificationen
dc.subjectmicrofluidic systemen
dc.subjectperipheral-blooden
dc.subjectpurificationen
dc.subjectchallengesen
dc.subjectenrichmenten
dc.subjectmoleculesen
dc.titleImmunomagnetic separation of tumor initiating cells by screening two surface markersen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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