Expression analysis and knockdown of two antennal odorant-binding protein genes in Aedes aegypti

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dc.contributor.authorSengul, Meryem S.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.departmentBiochemistryen
dc.date.accessioned2019-07-30T19:25:21Zen
dc.date.available2019-07-30T19:25:21Zen
dc.date.issued2010-10-05en
dc.description.abstractThe presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the host-seeking behavior of mosquitoes. It is important to establish a method in which a loss-of-function test can be performed to determine the possible role of these genes in olfaction. In this study, a double subgenomic Sindbis virus expression system was used to reduce the expression of two Obp genes in Aedes aegypti L (Diptera: Culicidae), AaegObp1 and AaegObp2. Quantitative real-time PCR analysis showed predominant expression of both genes in the female antennae, the primary olfactory tissue of mosquitoes. Moreover, at 11 days post virus-inoculation, the mRNA levels of AaegObp1 and AaegObp2 were significantly reduced in olfactory tissues of recombinant virus-inoculated female mosquitoes compared to that of controls by approximately 8 and 100-fold, respectively. These data suggest that the double subgenomic Sindbis virus expression system can be efficiently used to knockdown Obp gene expression in olfactory tissues of mosquitoes. We discuss the potential for a systematic analysis of the molecular players involved in mosquito olfaction using this newly developed technique. Such analysis will provide an important step to interfere with the hostseeking behavior of mosquitoes to prevent the transmission of diseases.en
dc.description.notesWe thank Drs. Kevin M. Myles and Zachary N. Adelman for providing the pTE/3'2J plasmid and leading us through the work with Sindbis viruses. We also thank Dr. Yumin Qi for assistance in cell culture and Thomas R. Saunders for rearing Ae. aegypti mosquitoes. This work was supported by National Institutes of Health Grant AI063252 to ZT.en
dc.description.sponsorshipNational Institutes of Health [AI063252]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1673/031.010.14131en
dc.identifier.eissn2250-2645en
dc.identifier.issn1536-2442en
dc.identifier.issue1en
dc.identifier.other171en
dc.identifier.pmid21062207en
dc.identifier.urihttp://hdl.handle.net/10919/92193en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherEntomological Society of Americaen
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectGene knockdownen
dc.subjectmosquitoen
dc.subjectolfactionen
dc.subjectRNAien
dc.subjectSindbis virusen
dc.titleExpression analysis and knockdown of two antennal odorant-binding protein genes in Aedes aegyptien
dc.title.serialJournal of Insect Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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