AnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactor

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dc.contributor.authorPerez-Gonzalez, Anaen
dc.contributor.authorJimenez-Vicente, Emilioen
dc.contributor.authorSalinero-Lanzarote, Alvaroen
dc.contributor.authorHarris, Derek F.en
dc.contributor.authorSeefeldt, Lance C.en
dc.contributor.authorDean, Dennis R.en
dc.date.accessioned2022-07-13T12:51:18Zen
dc.date.available2022-07-13T12:51:18Zen
dc.date.issued2022-05en
dc.description.abstractAzotobacter vinelandii produces three genetically distinct, but structurally and mechanistically similar nitrogenase isozymes designated as Mo-dependent, V-dependent, or Fe-only based on the heterometal contained within their associated active site cofactors. These catalytic cofactors, which provide the site for N-2 binding and reduction, are, respectively, designated as FeMo-cofactor, FeV-cofactor, and FeFe-cofactor. Fe-only nitrogenase is a poor catalyst for N-2 fixation, when compared to the Mo-dependent and V-dependent nitrogenases and is only produced when neither Mo nor V is available. Under conditions favoring the production of Fe-only nitrogenase a gene product designated AnfO preserves the fidelity of Fe-only nitrogenase by preventing the misincorporation of FeV-cofactor, which results in the accumulation of a hybrid enzyme that cannot reduce N-2. These results are interpreted to indicate that AnfO controls the fidelity of Fe-only nitrogenase maturation during the physiological transition from conditions that favor V-dependent nitrogenase utilization to Fe-only nitrogenase utilization to support diazotrophic growth.en
dc.description.notesBill and Melinda Gates Foundation, Grant/Award Number: BNF Cereals Phase II (OPP1143172) and BNF Cereals Phase III (INV--005889); U.S Department of Energy, Basic Energy Sciences, Grant/Award Number: DE--SC0010687en
dc.description.sponsorshipBill and Melinda Gates Foundation [OPP1143172]; BNF Cereals Phase III [INV-005889]; U.S Department of Energy, Basic Energy Sciences [DE-SC0010687]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/mmi.14890en
dc.identifier.eissn1365-2958en
dc.identifier.issn0950-382Xen
dc.identifier.issue5en
dc.identifier.pmid35220629en
dc.identifier.urihttp://hdl.handle.net/10919/111233en
dc.identifier.volume117en
dc.language.isoenen
dc.publisherWileyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAzotobacter vinelandiien
dc.subjectFeFe proteinen
dc.subjectFeV-cofactoren
dc.subjectmetalloproteinen
dc.subjectnitrogen fixationen
dc.subjectnitrogenaseen
dc.titleAnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactoren
dc.title.serialMolecular Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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