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Reprogramming macrophage orientation by microRNA 146b targeting transcription factor IRF5

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dc.contributor.authorPeng, Liangen
dc.contributor.authorZhang, Huien
dc.contributor.authorHao, Yuanyuanen
dc.contributor.authorXu, Feihongen
dc.contributor.authorYang, Jianjunen
dc.contributor.authorZhang, Ruihuaen
dc.contributor.authorLu, Gemingen
dc.contributor.authorZheng, Zihanen
dc.contributor.authorCui, Miaoen
dc.contributor.authorQi, Chen-Fengen
dc.contributor.authorChen, Chunen
dc.contributor.authorWang, Juanen
dc.contributor.authorHu, Yuanen
dc.contributor.authorWang, Dien
dc.contributor.authorPierce, Susanen
dc.contributor.authorLi, Liwuen
dc.contributor.authorXiong, Huabaoen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2019-08-28T14:20:19Zen
dc.date.available2019-08-28T14:20:19Zen
dc.date.issued2016-12en
dc.description.abstractThe regulation of macrophage orientation pathological conditions is important but still incompletely understood. Here, we show that IL-10 and Rag1 double knockout mice spontaneously develop colitis with dominant M1 macrophage phenotype, suggesting that IL-10 regulates macrophage orientation in inflammation. We demonstrate that IL-10 stimulation induced miR-146b expression, and that the expression of miR-146b was impaired in IL-10 deficient macrophages. Our data show that miR-146b targets IRF5, resulting in the regulation of macrophage activation. Furthermore, miR-146b deficient mice developed intestinal inflammation with enhanced M1 macrophage polarization. Finally, miR-146b mimic treatment significantly suppresses M1 macrophage activation and ameliorates colitis development in vivo. Collectively, the results suggest that IL-10 dependent miR-146b plays an important role in the modulation of M1 macrophage orientation. (C) 2016 The Authors. Published by Elsevier B.V.en
dc.description.notesH.X. was supported by the NIH funding (RO1AI104688).en
dc.description.sponsorshipNIH [RO1AI104688]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.ebiom.2016.10.041en
dc.identifier.issn2352-3964en
dc.identifier.pmid27825654en
dc.identifier.urihttp://hdl.handle.net/10919/93279en
dc.identifier.volume14en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectInterleukin 10en
dc.subjectmiR-146ben
dc.subjectMacrophageen
dc.subjectCRISPR/Cas9en
dc.subjectColitisen
dc.titleReprogramming macrophage orientation by microRNA 146b targeting transcription factor IRF5en
dc.title.serialEBioMedicineen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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