Biological watermelon (Citrullus lanatus L.) seed treatments for control of Acidovorax citrulli

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dc.contributor.authorKlein, Rachelen
dc.contributor.committeechairWelbaum, Gregory E.en
dc.contributor.committeememberBaudoin, Antonius B.en
dc.contributor.committeememberWestwood, James H.en
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen
dc.date.accessioned2020-06-03T17:16:22Zen
dc.date.available2020-06-03T17:16:22Zen
dc.date.issued2020-06-03en
dc.description.abstractAcidovorax citrulli is a seedborne pathogen responsible for bacterial fruit blotch (BFB), an economically important disease in melon and watermelon throughout the world. BFB is highly virulent and in affected fields can cause yield reduction of up to 95%, which has resulted in over $100,000 in losses to melon growers in some cases. The efficacy of green tea as an antimicrobial seed treatment against A. citrulli was tested. Watermelon seeds were treated with green tea after inoculation with transgenic A. citrulli expressing green fluorescent protein (GFP). Forty five percent of watermelon seedlings inoculated with a high level (OD600:1.0, ~8 x 108 cells/ml) of A. citrulli displayed GFP in their cotyledons. When these seeds were treated with green tea, only 11.2% displayed GFP in their cotyledons. None of the treated watermelon seedlings inoculated with a low level (OD600:0.001, ~8 x 105 cells/ml) of A. citrulli displayed GFP in their cotyledons. Green tea treatments effectively controlled the disease when administered as a liquid to infected watermelon seeds. Green tea has potential as an effective commercial treatment for pericarp infected seeds that could also be used by growers participating in the National Organic Program.en
dc.description.degreeMALSen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/98684en
dc.language.isoenen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.cabtAcidovorax citrullien
dc.subject.cabtbacterial fruit blotchen
dc.subject.cabtcucurbiten
dc.subject.cabtseed treatmenten
dc.subject.cabtgreen teaen
dc.subject.cabtorganic agricultureen
dc.titleBiological watermelon (Citrullus lanatus L.) seed treatments for control of Acidovorax citrullien
dc.typeMaster's projecten
dc.type.dcmitypeTexten
thesis.degree.disciplinePlant Science and Pest Managementen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Agricultural and Life Sciencesen

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