Resident Microbiome Disruption with Antibiotics Enhances Virulence of a Colonizing Pathogen

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dc.contributor.authorThomason, Courtney A.en
dc.contributor.authorMullen, Nathanen
dc.contributor.authorBelden, Lisa K.en
dc.contributor.authorMay, Meghanen
dc.contributor.authorHawley, Dana M.en
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2019-01-02T15:41:30Zen
dc.date.available2019-01-02T15:41:30Zen
dc.date.issued2017-11-23en
dc.description.abstractThere is growing evidence that symbiotic microbes play key roles in host defense, but less is known about how symbiotic microbes mediate pathogen-induced damage to hosts. Here, we use a natural wildlife disease system, house finches and the conjunctival bacterial pathogen Mycoplasma gallisepticum (MG), to experimentally examine the impact of the ocular microbiome on host damage and pathogen virulence factors during infection. We disrupted the ocular bacterial community of healthy finches using an antibiotic that MG is intrinsically resistant to, then inoculated antibiotic and sham-treated birds with MG. House finches with antibiotic-disrupted ocular microbiomes had more severe MG-induced conjunctival inflammation than birds with unaltered microbiomes, even after accounting for differences in conjunctival MG load. Furthermore, MG cultures from finches with disrupted microbiomes had increased sialidase enzyme and cytadherence activity, traits associated with enhanced virulence in Mycoplasmas, relative to isolates from sham-treated birds. Variation in sialidase activity and cytadherence among isolates was tightly linked with degree of tissue inflammation in hosts, supporting the consideration of these traits as virulence factors in this system. Overall, our results suggest that microbial dysbiosis can result in enhanced virulence of colonizing pathogens, with critical implications for the health of wildlife, domestic animals, and humans.en
dc.description.notesThis work was funded by NIH grant 5R01GM105245 (to DMH) as part of the joint NIH-NSF-USDA Ecology and Evolution of Infectious Diseases program and NSF grant DEB-1136640 to LKB. We thank Laila Kirkpatrick, Jeni Walke, and Courtney Pearson for technical assistance, and Ariel Leon, Sahnzi Moyers, Matt Aberle, Eddie Schuler, Natalie Bales, Courtney Youngbar, Ashlyn Garnish, Sarah Taylor, Morgan Villa, and Michal Vinkler for assistance with sample collection. Finally, we thank David Ley for providing isolates and the rest of the House Finch project team for useful feedback.en
dc.description.sponsorshipNIH as part of the joint NIH-NSF-USDA Ecology and Evolution of Infectious Diseases program [5R01GM105245]; NSF grant [DEB-1136640]en
dc.format.extent8 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-017-16393-3en
dc.identifier.issn2045-2322en
dc.identifier.other16177en
dc.identifier.pmid29170421en
dc.identifier.urihttp://hdl.handle.net/10919/86544en
dc.identifier.volume7en
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmycoplasma-gallisepticum infectionen
dc.subjectin-house finchesen
dc.subjectimmune-systemen
dc.subjectdiseaseen
dc.subjectbacteriaen
dc.subjectsusceptibilityen
dc.subjectconjunctivitisen
dc.subjectcytadherenceen
dc.subjecttransmissionen
dc.subjectsurvivalen
dc.titleResident Microbiome Disruption with Antibiotics Enhances Virulence of a Colonizing Pathogenen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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