A correlation force spectrometer for single molecule measurements under tensile load

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dc.contributorVirginia Tech. Department of Chemical Engineeringen
dc.contributorVirginia Tech. Department of Mechanical Engineeringen
dc.contributor.authorRadiom, Miladen
dc.contributor.authorHonig, Christopher D. F.en
dc.contributor.authorWalz, John Y.en
dc.contributor.authorPaul, Mark R.en
dc.contributor.authorDucker, William A.en
dc.contributor.departmentChemical Engineeringen
dc.date.accessed2015-04-24en
dc.date.accessioned2015-05-05T15:41:58Zen
dc.date.available2015-05-05T15:41:58Zen
dc.date.issued2013-01-07en
dc.description.abstractThe dynamical-mechanical properties of a small region of fluid can be measured using two closely spaced thermally stimulated micrometer-scale cantilevers. We call this technique correlation force spectroscopy (CFS). We describe an instrument that is designed for characterizing the extensional properties of polymer molecules that straddle the gap between the two cantilevers and use it to measure the stiffness and damping (molecular friction) of a dextran molecule. The device is based on a commercial atomic force microscope, into which we have incorporated a second antiparallel cantilever. The deflection of each cantilever is measured in the frequency range dc-1 MHz and is used to generate the cross-correlation at equilibrium. The main advantage of cross-correlation measurements is the reduction in thermal noise, which sets a fundamental noise limit to force resolution. We show that the thermal noise in our cross-correlation measurements is less than one third of the value for single-cantilever force microscopy. The dynamics of the cantilever pair is modeled using the deterministic motion of a harmonic oscillator initially displaced from equilibrium, which yields the equilibrium auto and cross-correlations in cantilever displacement via the fluctuation-dissipation theorem. Fitted parameters from the model (stiffness and damping) are used to characterize the fluid at equilibrium, including any straddling molecules. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4772646]en
dc.description.sponsorshipNational Science Foundation (U.S.) - Award Number CBET-0959228en
dc.description.sponsorshipVirginia Techen
dc.format.extent11 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRadiom, Milad, Honig, Christopher D. F., Walz, John Y., Paul, Mark R., Ducker, William A. (2013). A correlation force spectrometer for single molecule measurements under tensile load. Journal of Applied Physics, 113(1). doi: 10.1063/1.4772646en
dc.identifier.doihttps://doi.org/10.1063/1.4772646en
dc.identifier.issn0021-8979en
dc.identifier.urihttp://hdl.handle.net/10919/52004en
dc.identifier.urlhttp://scitation.aip.org/content/aip/journal/jap/113/1/10.1063/1.4772646en
dc.language.isoen_USen
dc.publisherAmerican Institute of Physicsen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAtomic force microscopyen
dc.subjectThermal noiseen
dc.subjectPolymersen
dc.subjectSingle molecule spectroscopyen
dc.subjectMolecular fluctuationsen
dc.titleA correlation force spectrometer for single molecule measurements under tensile loaden
dc.title.serialJournal of Applied Physicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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