Microfluidic LC Device with Orthogonal Sample Extraction for On-Chip MALDI-MS Detection

dc.contributor.authorLazar, Iuliana M.en
dc.contributor.authorKabulski, Jarod L.en
dc.contributor.departmentBiological Sciencesen
dc.date.accessed2015-04-17en
dc.date.accessioned2015-04-20T21:04:11Zen
dc.date.available2015-04-20T21:04:11Zen
dc.date.issued2013-03-20en
dc.description.abstractA microfluidic device that enables on-chip matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) detection for liquid chromatography (LC) separations is described. The device comprises an array of functional elements to carry out LC separations, integrates a novel microchip-MS interface to facilitate the orthogonal transposition of the microfluidic LC channel into an array of reservoirs, and enables sensitive MALDI-MS detection directly from the chip. Essentially, the device provides a snapshot MALDI-MS map of the content of the separation channel present on the chip. The detection of proteins with biomarker potential from MCF10A breast epithelial cell extracts, and detection limits in the low fmol range, are demonstrated. In addition, the design of the novel LC-MALDI-MS chip entices the promotion of a new concept for performing sample separations within the limited time-frame that accompanies the dead-volume of a separation channel.en
dc.description.notesSupplementary information is included in separate filesen
dc.description.sponsorshipNational Institutes of Health. National Cancer Institute - Award Number R21CA126669-01A1en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/vnd.ms-excelen
dc.format.mimetypeimage/jpegen
dc.identifier.citationLazar, I. M., & Kabulski, J. L. (2013). Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection. Lab on a Chip, 13(11), 2055-2065. doi: 10.1039/C3LC50190Fen
dc.identifier.doihttps://doi.org/10.1039/C3LC50190Fen
dc.identifier.issn1473-0197en
dc.identifier.urihttp://hdl.handle.net/10919/51708en
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc50190fen
dc.language.isoen_USen
dc.publisherThe Royal Society of Chemistryen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectMass spectrometryen
dc.subjectLiquid chromatographyen
dc.subjectMatrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)en
dc.titleMicrofluidic LC Device with Orthogonal Sample Extraction for On-Chip MALDI-MS Detectionen
dc.title.serialLab on a Chipen
dc.typeArticle - Refereeden
dc.typeDataseten
dc.typeImageen
dc.type.dcmitypeTexten
dc.type.dcmitypeDataseten
dc.type.dcmitypeImageen

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