Renewable fatty acid ester production in Clostridium

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dc.contributor.authorFeng, Junen
dc.contributor.authorZhang, Jieen
dc.contributor.authorMa, Yuechaoen
dc.contributor.authorFeng, Yimingen
dc.contributor.authorWang, Shangjunen
dc.contributor.authorGuo, Naen
dc.contributor.authorWang, Haijiaoen
dc.contributor.authorWang, Pixiangen
dc.contributor.authorJimenez-Bonilla, Pabloen
dc.contributor.authorGu, Yanyanen
dc.contributor.authorZhou, Junpingen
dc.contributor.authorZhang, Zhong-Tianen
dc.contributor.authorCao, Mingfengen
dc.contributor.authorJiang, Dien
dc.contributor.authorWang, Shuningen
dc.contributor.authorLiu, Xian-Weien
dc.contributor.authorShao, Zengyien
dc.contributor.authorBorovok, Ilyaen
dc.contributor.authorHuang, Haiboen
dc.contributor.authorWang, Yien
dc.date.accessioned2022-04-07T18:36:17Zen
dc.date.available2022-04-07T18:36:17Zen
dc.date.issued2021-07-16en
dc.description.abstractBioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3g/L butyl acetate and 1.6g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses. Esters can be used as fuels and specialty chemicals for food flavoring, cosmetic and pharmaceutical industries. Here, the authors systematically engineer clostridia, including discovery and deletion of prophages to increase strain stability, for the production of butyl acetate and butyl butyrate from corn stover at low cost.en
dc.description.notesThis work was supported by the US Department of Energy's Office of Energy Efficiency and Renewable Energy under Award DE-EE0008483 (Co-Optima), the Agriculture and Food Research Initiative Competitive Grant no. 2018-67021-27715 from the USDA National Institute of Food and Agriculture (NIFA), the Auburn University Intramural Grants Program (IGP), the USDA-NIFA Hatch project (ALA014-1017025), and the Alabama Agricultural Experiment Station. We thank Dr. Hans Blaschek (University of Illinois at Urbana-Champaign) for providing plasmids pYW34 and pTJ1, Dr. Nigel Minton (University of Nottingham, UK) for providing pMTL series of plasmids, Dr. Cong T Trinh (University of Tennessee) for providing plasmids pDL001, pDL004 and pDL006, and Dr. Mike Young (Aberystwyth University, UK) for providing E. coli CA434. We thank Ms. Sheng Dong for constructing C. pasteurianum SD-1, and thank Drs. Michael Pyne and C. Perry Chou (University of Waterloo, Canada) for providing research materials and technical assistance for working on C. pasteurianum. We thank Dr. Daniel Schell (National Renewable Energy Laboratory) for providing the corn stover hydrolysates. We also thank Drs. Raymond P. Henry, Michael E. Miller, Nannan Liu, Evert C. Duin, and Brendan Higgins (all from Auburn University) for allowing us to access their equipment.en
dc.description.sponsorshipUS Department of Energy's Office of Energy Efficiency and Renewable EnergyUnited States Department of Energy (DOE) [DE-EE0008483]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture (NIFA) [2018-67021-27715]; Auburn University Intramural Grants Program (IGP); USDA-NIFA Hatch project [ALA014-1017025]; Alabama Agricultural Experiment Stationen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41467-021-24038-3en
dc.identifier.issn2041-1723en
dc.identifier.issue1en
dc.identifier.other4368en
dc.identifier.pmid34272383en
dc.identifier.urihttp://hdl.handle.net/10919/109597en
dc.identifier.volume12en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleRenewable fatty acid ester production in Clostridiumen
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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