Nix is a male-determining factor in the Asian tiger mosquito Aedes albopictus

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dc.contributor.authorLiu, Peiwenen
dc.contributor.authorJin, Binbinen
dc.contributor.authorLi, Xiaocongen
dc.contributor.authorZhao, Yijieen
dc.contributor.authorGu, Jinbaoen
dc.contributor.authorBiedler, James K.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorChen, Xiaoguangen
dc.contributor.departmentBiochemistryen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2021-01-07T15:04:13Zen
dc.date.available2021-01-07T15:04:13Zen
dc.date.issued2020-03en
dc.description.abstractThe initial signal that governs sex determination is highly variable among insects. A homolog of Nix, the male-determining factor in Aedes aegypti, was previously found in the Asian tiger mosquito Ae. albopictus. Here we show that the Ae. albopictus Nix (AalNix) is more complex in gene structure and splice isoforms than its Ae. aegypti homolog (AaeNix). AalNix shows a similar transcription profile compared to AaeNix. CRISPR/Cas9mediated knockouts of AalNix in vivo and in the Ae. albopictus C6/36 cells lead to a shift of dsx and fru splicing towards the female isoforms. G(o) knockout males showed feminization and deformities including feminized antennae, absence or partial absence of gonocoxites, gonostyli, testes and accessory glands, and the formation of ovaries. Despite similar to 70 MY of divergence, Nix functions as a conserved male-determining factor in the two most important arboviral vectors, namely Ae. aegypti and Ae. albopictus.en
dc.description.notesThis work is supported by a combination of funding from the: National Natural Science Foundation of China of China (81420108024, 81829004), National Institutes of Health, USA (AI136850, AI123338) and Natural Science Foundation of Guangdong Province (2014A030312016). We thank J.L Liu for pAc-sgRNA-Cas9, which was obtained through addgeneg.org.en
dc.description.sponsorshipNational Natural Science Foundation of China of ChinaNational Natural Science Foundation of China (NSFC) [81420108024, 81829004]; National Institutes of Health, USAUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [AI136850, AI123338]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2014A030312016]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.ibmb.2019.103311en
dc.identifier.eissn1879-0240en
dc.identifier.issn0965-1748en
dc.identifier.other103311en
dc.identifier.pmid31901476en
dc.identifier.urihttp://hdl.handle.net/10919/101769en
dc.identifier.volume118en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectM-factoren
dc.subjectSex-determinationen
dc.subjectCRSPR-Cas9en
dc.subjectFeminizeden
dc.titleNix is a male-determining factor in the Asian tiger mosquito Aedes albopictusen
dc.title.serialInsect Biochemistry and Molecular Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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