An anomalous type IV secretion system in Rickettsia is evolutionarily conserved

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dc.contributor.authorGillespie, Joseph J.en
dc.contributor.authorAmmerman, Nicole C.en
dc.contributor.authorDreher-Lesnick, Sheila M.en
dc.contributor.authorRahman, Sayeeduren
dc.contributor.authorWorley, Micah J.en
dc.contributor.authorSetubal, João C.en
dc.contributor.authorSobral, Brunoen
dc.contributor.authorAzad, Abdu F.en
dc.date.accessed2014-05-01en
dc.date.accessioned2014-06-17T20:12:03Zen
dc.date.available2014-06-17T20:12:03Zen
dc.date.issued2009-03-12en
dc.description.abstractBackground: Bacterial type IV secretion systems (T4SSs) comprise a diverse transporter family functioning in conjugation, competence, and effector molecule (DNA and/or protein) translocation. Thirteen genome sequences from Rickettsia, obligate intracellular symbionts/pathogens of a wide range of eukaryotes, have revealed a reduced T4SS relative to the Agrobacterium tumefaciens archetype (vir). However, the Rickettsia T4SS has not been functionally characterized for its role in symbiosis/virulence, and none of its substrates are known. Results: Superimposition of T4SS structural/functional information over previously identified Rickettsia components implicate a functional Rickettsia T4SS. virB4, virB8 and virB9 are duplicated, yet only one copy of each has the conserved features of similar genes in other T4SSs. An extraordinarily duplicated VirB6 gene encodes five hydrophobic proteins conserved only in a short region known to be involved in DNA transfer in A. tumefaciens. virB1, virB2 and virB7 are newly identified, revealing a Rickettsia T4SS lacking only virB5 relative to the vir archetype. Phylogeny estimation suggests vertical inheritance of all components, despite gene rearrangements into an archipelago of five islets. Similarities of Rickettsia VirB7/ VirB9 to ComB7/ComB9 proteins of e-proteobacteria, as well as phylogenetic affinities to the Legionella lvh T4SS, imply the Rickettsiales ancestor acquired a vir-like locus from distantly related bacteria, perhaps while residing in a protozoan host. Modern modifications of these systems likely reflect diversification with various eukaryotic host cells. Conclusion: We present the rvh (Rickettsiales vir homolog) T4SS, an evolutionary conserved transporter with an unknown role in rickettsial biology. This work lays the foundation for future laboratory characterization of this system, and also identifies the Legionella lvh T4SS as a suitable genetic model.en
dc.description.sponsorshipThe project described was supported by Award Numbers R01AI017828 and R01AI59118 from the National Institute of Allergy And Infectious Diseases (NIAID) to AFA, and through NIAID contract HHSN266200400035C to BSS. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIAID or the National Institutes of Healthen
dc.identifier.citationGillespie JJ, Ammerman NC, Dreher-Lesnick SM, Rahman MS, Worley MJ, et al. (2009) An Anomalous Type IV Secretion System in Rickettsia Is Evolutionarily Conserved. PLoS ONE 4(3): e4833. doi:10.1371/journal.pone.0004833en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0004833en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/48969en
dc.identifier.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0004833en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAgrobacterium tumefaciensen
dc.subjectAgrobacterium tumefaciensen
dc.subjectGenome evolutionen
dc.subjectMultiple alignment calculationen
dc.subjectPhylogenetic analysisen
dc.subjectRickettsiaen
dc.subjectSequence alignmenten
dc.subjectSequence motif analysisen
dc.titleAn anomalous type IV secretion system in Rickettsia is evolutionarily conserveden
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden

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