Focusing of mammalian cells under an ultrahigh pH gradient created by unidirectional electropulsation in a confined microchamber

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dc.contributorVirginia Tech. Department of Chemical Engineeringen
dc.contributorVirginia Tech-Wake Forest University School of Biomedical Engineering and Sciencesen
dc.contributorVirginia Tech. Virginia Bioinformatics Instituteen
dc.contributorVirginia Tech. Department of Biological Sciencesen
dc.contributor.authorLoufakis, Despina N.en
dc.contributor.authorCao, Zhenningen
dc.contributor.authorMa, Saien
dc.contributor.authorMittelman, Daviden
dc.contributor.authorLu, Changen
dc.contributor.departmentChemical Engineeringen
dc.date.accessed2015-04-16en
dc.date.accessioned2015-04-20T21:59:29Zen
dc.date.available2015-04-20T21:59:29Zen
dc.date.issued2014-06-09en
dc.description.abstractThe transport and manipulation of cells in microfluidic structures are often critically required in cellular analysis. Cells typically make consistent movement in a dc electric field in a single direction, due to their electrophoretic mobility or electroosmotic flow or the combination of the two. Here we demonstrate that mammalian cells focus to the middle of a closed microfluidic chamber under the application of unidirectional direct current pulses. With experimental and computational data, we show that under the pulses electrochemical reactions take place in the confined microscale space and create an ultrahigh and nonlinear pH gradient (~2 orders of magnitude higher than the ones in protein isoelectric focusing) at the middle of the chamber. The varying local pH affects the cell surface charge and the electrophoretic mobility, leading to focusing in free solution. Our approach provides a new and simple method for focusing and concentrating mammalian cells at the microscale.en
dc.description.notesSupplementary information is included in separate filesen
dc.description.sponsorshipNational Science Foundation (U.S.). Chemical, Bioengineering, Environmental and Transport Systems - 1016547en
dc.description.sponsorshipNational Institutes of Health. National Cancer Institute - R21CA174577en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypevideo/avien
dc.identifier.citationLoufakis, D. N., Cao, Z., Ma, S., Mittelman, D., & Lu, C. (2014). Focusing of mammalian cells under an ultrahigh pH gradient created by unidirectional electropulsation in a confined microchamber. Chemical Science, 5(8), 3331-3337. doi: 10.1039/C4SC00319Een
dc.identifier.doihttps://doi.org/10.1039/C4SC00319Een
dc.identifier.issn2041-6520en
dc.identifier.urihttp://hdl.handle.net/10919/51717en
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2014/sc/c4sc00319een
dc.language.isoen_USen
dc.publisherThe Royal Society of Chemistryen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectProteinsen
dc.subjectPeptidesen
dc.subjectMicrofluidic structuresen
dc.subjectMammalian cellsen
dc.subjectCOMSOL Multiphysicsen
dc.titleFocusing of mammalian cells under an ultrahigh pH gradient created by unidirectional electropulsation in a confined microchamberen
dc.title.serialChemical Scienceen
dc.typeArticle - Refereeden
dc.typeVideoen
dc.type.dcmitypeTexten
dc.type.dcmitypeImageen
dc.type.dcmitypeMovingImageen

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