Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma

Simple item page
dc.contributorVirginia Techen
dc.contributor.authorMorazzani, Elaine M.en
dc.contributor.authorWiley, Michael R.en
dc.contributor.authorMurreddu, Marta G.en
dc.contributor.authorAdelman, Zach N.en
dc.contributor.authorMyles, Kevin M.en
dc.date.accessioned2014-07-01T18:58:25Zen
dc.date.available2014-07-01T18:58:25Zen
dc.date.issued2012-01-05en
dc.description.abstractThe natural maintenance cycles of many mosquito-borne pathogens require establishment of persistent non-lethal infections in the invertebrate host. The mechanism by which this occurs is not well understood, but we have previously shown that an antiviral response directed by small interfering RNAs (siRNAs) is important in modulating the pathogenesis of alphavirus infections in the mosquito. However, we report here that infection of mosquitoes with an alphavirus also triggers the production of another class of virus-derived small RNAs that exhibit many similarities to ping-pong-dependent piwiinteracting RNAs (piRNAs). However, unlike ping-pong-dependent piRNAs that have been described previously from repetitive elements or piRNA clusters, our work suggests production in the soma. We also present evidence that suggests virus-derived piRNA-like small RNAs are capable of modulating the pathogenesis of alphavirus infections in dicer-2 null mutant mosquito cell lines defective in viral siRNA production. Overall, our results suggest that a non-canonical piRNA pathway is present in the soma of vector mosquitoes and may be acting redundantly to the siRNA pathway to target alphavirus replication.en
dc.description.sponsorshipThe project described was supported by Grant Number AI077726 from NIAID. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.citationMorazzani EM, Wiley MR, Murreddu MG, Adelman ZN, Myles KM (2012) Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma. PLoS Pathog 8(1): e1002470. doi:10.1371/journal.ppat.1002470en
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1002470en
dc.identifier.issn1553-7374en
dc.identifier.urihttp://hdl.handle.net/10919/49255en
dc.identifier.urlhttp://www.plospathogens.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.ppat.1002470en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectAntiviralsen
dc.subjectChikungunya infectionen
dc.subjectImmune responseen
dc.subjectMosquitoesen
dc.subjectRNA sequencingen
dc.subjectRNA virusesen
dc.subjectSmall interfering RNAsen
dc.subjectdsRNA virusesen
dc.titleProduction of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Somaen
dc.title.serialPLoS Pathogensen
dc.typeArticle - Refereeden

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.ppat.1002470.pdf
Size:
1.16 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Scholarly Works, Fralin Life Sciences Institute