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Differential Stress Transcriptome Landscape of Historic and Recently Emerged Hypervirulent Strains of Clostridium difficile Strains Determined Using RNA-seq

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dc.contributor.authorScaria, Joyen
dc.contributor.authorMao, Chunhongen
dc.contributor.authorChen, Jenn-Weien
dc.contributor.authorMcDonough, Sean P.en
dc.contributor.authorSobral, Brunoen
dc.contributor.authorChang, Yung-Fuen
dc.date.accessed2014-05-07en
dc.date.accessioned2014-06-17T20:12:09Zen
dc.date.available2014-06-17T20:12:09Zen
dc.date.issued2014-11-07en
dc.description.abstractC. difficile is the most common cause of nosocomial diarrhea in North America and Europe. Genomes of individual strains of C. difficile are highly divergent. To determine how divergent strains respond to environmental changes, the transcriptomes of two historic and two recently isolated hypervirulent strains were analyzed following nutrient shift and osmotic shock. Illumina based RNA-seq was used to sequence these transcriptomes. Our results reveal that although C. difficile strains contain a large number of shared and strain specific genes, the majority of the differentially expressed genes were core genes. We also detected a number of transcriptionally active regions that were not part of the primary genome annotation. Some of these are likely to be small regulatory RNAs.en
dc.description.sponsorshipThis work was partially supported by a subcontract (project no. 62078/A001) from the Virginia Tech, which has been funded from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900040C, a grant (NYCV-478820) from the USDA Animal Health and Disease Research Program and a grant (NYC-478499) from the USDA formula fund. No additional external funding was received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.citationScaria J, Mao C, Chen J-W, McDonough SP, Sobral B, Chang Y-F. Differential Stress Transcriptome Landscape of Historic and Recently Emerged Hypervirulent Strains of Clostridium difficile Strains Determined Using RNA-seq. PLoS One. 2013;8(11):e78489. doi:10.1371%2Fjournal.pone.0078489en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0078489en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/49006en
dc.identifier.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078489en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectClostridium difficileen
dc.subjectGene expressionen
dc.subjectGenonmic databasesen
dc.subjectNutrientsen
dc.subjectOsmotic shocken
dc.subjectRNA sequencingen
dc.subjectToinsen
dc.subjectTranscriptome analysisen
dc.titleDifferential Stress Transcriptome Landscape of Historic and Recently Emerged Hypervirulent Strains of Clostridium difficile Strains Determined Using RNA-seqen
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden

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