Analysis of Myxococcus xanthus Vegetative Biofilms With Microtiter Plates

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dc.contributor.authorDye, Keane J.en
dc.contributor.authorYang, Zhaominen
dc.date.accessioned2022-06-28T15:16:12Zen
dc.date.available2022-06-28T15:16:12Zen
dc.date.issued2022-04-29en
dc.description.abstractThe bacterium Myxococcus xanthus forms both developmental and vegetative types of biofilms. While the former has been studied on both agar plates and submerged surfaces, the latter has been investigated predominantly on agar surfaces as swarming colonies. Here we describe the development of a microplate-based assay for the submerged biofilms of M. xanthus under vegetative conditions. We examined the impacts of inoculation, aeration, and temperature to optimize the conditions for the assay. Aeration was observed to be critical for the effective development of submerged biofilms by M. xanthus, an obligate aerobic bacterium. In addition, temperature plays an important role in the development of M. xanthus submerged biofilms. It is well established that the formation of submerged biofilms by many bacteria requires both exopolysaccharide (EPS) and the type IV pilus (T4P). EPS constitutes part of the biofilm matrix that maintains and organizes bacterial biofilms while the T4P facilitates surface attachment as adhesins. For validation, we used our biofilm assay to examine a multitude of M. xanthus strains with various EPS and T4P phenotypes. The results indicate that the levels of EPS, but not of piliation, positively correlate with submerged biofilm formation in M. xanthus.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2022.894562en
dc.identifier.eissn1664-302Xen
dc.identifier.other894562en
dc.identifier.pmid35572678en
dc.identifier.urihttp://hdl.handle.net/10919/110959en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMyxococcus xanthusen
dc.subjectvegetative biofilmsen
dc.subjectexopolysaccharide (EPS)en
dc.subjecttype IV pilus (T4P)en
dc.subjectmicroplate assayen
dc.titleAnalysis of Myxococcus xanthus Vegetative Biofilms With Microtiter Platesen
dc.title.serialFrontiers in Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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