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Fluid mechanics approach to analyzing collagen fiber organization

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dc.contributor.authorSalazar Coariti, Adriana C.en
dc.contributor.authorFabien, Maurice S.en
dc.contributor.authorGuzman, Johnnyen
dc.contributor.authorMcGuire, Jeffrey A.en
dc.contributor.authorDe Vita, Raffaellaen
dc.contributor.authorToussaint, Kimani C.en
dc.date.accessioned2022-08-30T14:23:58Zen
dc.date.available2022-08-30T14:23:58Zen
dc.date.issued2022-01-01en
dc.description.abstractSignificance: The spatial organization of collagen fibers has been used as a biomarker for assessing injury and disease progression. However, quantifying this organization for complex structures is challenging. Aim: To quantify and classify complex collagen fiber organizations. Approach: Using quantitative second-harmonic generation (SHG) microscopy, we show that collagen-fiber orientation can be viewed as pseudovector fields. Subsequently, we analyze them using fluid mechanic metrics, such as energy U, enstrophy E, and tortuosity tau. Results: We show that metrics used in fluid mechanics for analyzing fluid flow can be adapted to analyze complex collagen fiber organization. As examples, we consider SHG images of collagenous tissue for straight, wavy, and circular fiber structures. Conclusions: The results of this study show the utility of the chosen metrics to distinguish diverse and complex collagen organizations. We find that the distribution of values for E and U increases with collagen fiber disorganization, where they divide between low and high values corresponding to uniformly aligned fibers and disorganized collagen fibers, respectively. We also confirm that the values of tau cluster around 1 when the fibers are straight, and the range increases up to 1.5 when wavier fibers are present. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1117/1.JBO.27.1.016503en
dc.identifier.eissn1560-2281en
dc.identifier.issn1083-3668en
dc.identifier.issue1en
dc.identifier.other16503en
dc.identifier.pmid35102730en
dc.identifier.urihttp://hdl.handle.net/10919/111666en
dc.identifier.volume27en
dc.language.isoenen
dc.publisherSPIEen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectvector fieldsen
dc.subjectcollagenen
dc.subjectsecond-harmonic generationen
dc.subjectwavinessen
dc.subjectfluid mechanicsen
dc.titleFluid mechanics approach to analyzing collagen fiber organizationen
dc.title.serialJournal of Biomedical Opticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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