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Identifying the diet of a declining prairie grouse using DNA metabarcoding

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dc.contributor.authorSullins, Daniel S.en
dc.contributor.authorHaukos, David A.en
dc.contributor.authorCraine, Joseph M.en
dc.contributor.authorLautenbach, Joseph M.en
dc.contributor.authorRobinson, Samantha G.en
dc.contributor.authorLautenbach, Jonathan D.en
dc.contributor.authorKraft, John D.en
dc.contributor.authorPlumb, Reid T.en
dc.contributor.authorReitz, Jonathan H.en
dc.contributor.authorSandercock, Brett K.en
dc.contributor.authorFierer, Noahen
dc.contributor.departmentFish and Wildlife Conservationen
dc.date.accessioned2020-07-09T12:28:27Zen
dc.date.available2020-07-09T12:28:27Zen
dc.date.issued2018-07en
dc.description.abstractDiets during critical brooding and winter periods likely influence the growth of Lesser Prairie-Chicken (Tympanuchus pallidicinctus) populations. During the brooding period, rapidly growing Lesser Prairie-Chicken chicks have high calorie demands and are restricted to foods within immediate surroundings. For adults and juveniles during cold winters, meeting thermoregulatory demands with available food items of limited nutrient content may be challenging. Our objective was to determine the primary animal and plant components of Lesser Prairie-Chicken diets among native prairie, cropland, and Conservation Reserve Program (CRP) fields in Kansas and Colorado, USA, during brooding and winter using a DNA metabarcoding approach. Lesser Prairie-Chicken fecal samples (n = 314) were collected during summer 2014 and winter 2014-2015, DNA was extracted, amplified, and sequenced. A region of the cytochrome oxidase I (COI) gene was sequenced to determine the arthropod component of the diet, and a portion of the trnL intron region was used to determine the plant component. Relying on fecal DNA to quantify dietary composition, as opposed to traditional visual identification of gut contents, revealed a greater proportion of soft-bodied arthropods than previously recorded. Among 80 fecal samples for which threshold arthropod DNA reads were obtained, 35% of the sequences were most likely from Lepidoptera, 26% from Orthoptera, 14% from Araneae, 13% from Hemiptera, and 12% from other orders. Plant sequences from 137 fecal samples were composed of species similar to Ambrosia (27%), followed by species similar to Lactuca or Taraxacum (10%), Medicago (6%), and Triticum (5%). Forbs were the predominant (>50% of reads) plant food consumed during both brood rearing and winter. The importance both of native forbs and of a broad array of arthropods that rely on forbs suggests that disturbance regimes that promote forbs may be crucial in providing food for Lesser Prairie-Chickens in the northern portion of their distribution.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesFunding for the project was provided by Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant KS W-73-R-3); USDA Farm Services CRP Monitoring, Assessment, and Evaluation (12-IA-MRE CRP TA#7, KSCFWRU RWO 62); and USDA Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative.en
dc.description.sponsorshipKansas Wildlife, Parks, and Tourism [KS W-73-R-3]; USDA Farm Services CRP Monitoring, Assessment, and Evaluation [12-IA-MRE CRP TA#7, KSCFWRU RWO 62]; USDA Natural Resources Conservation Service, Lesser Prairie-Chicken Initiativeen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1642/AUK-17-199.1en
dc.identifier.eissn1938-4254en
dc.identifier.issn0004-8038en
dc.identifier.issue3en
dc.identifier.urihttp://hdl.handle.net/10919/99315en
dc.identifier.volume135en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectarthropodsen
dc.subjectdieten
dc.subjectDNA metabarcodingen
dc.subjectforagingen
dc.subjectforbsen
dc.subjectgrasslandsen
dc.subjectgrouseen
dc.subjectinvertebratesen
dc.subjectLesser Prairie-Chickenen
dc.subjectTympanuchus pallidicinctusen
dc.titleIdentifying the diet of a declining prairie grouse using DNA metabarcodingen
dc.title.serialAuken
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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