Metagenomic Analysis of a Tropical Composting Operation at the Sao Paulo Zoo Park Reveals Diversity of Biomass Degradation Functions and Organisms

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dc.contributor.authorMartins, Layla Farageen
dc.contributor.authorAntunes, Luciana Principalen
dc.contributor.authorPascon, Renata C.en
dc.contributor.authorFranco de Oliveira, Julio Cezaren
dc.contributor.authorDigiampietri, Luciano A.en
dc.contributor.authorBarbosa, Deibsen
dc.contributor.authorPeixoto, Bruno Malveiraen
dc.contributor.authorVallim, Marcelo A.en
dc.contributor.authorViana-Niero, Cristinaen
dc.contributor.authorOstroski, Eric H.en
dc.contributor.authorTelles, Guilherme P.en
dc.contributor.authorDias, Zanonien
dc.contributor.authorda Cruz, Joao Batistaen
dc.contributor.authorJuliano, Luizen
dc.contributor.authorVerjovski-Almeida, Sergioen
dc.contributor.authorda Silva, Aline M.en
dc.contributor.authorSetubal, Joao Carlosen
dc.date.accessioned2018-10-22T14:05:15Zen
dc.date.available2018-10-22T14:05:15Zen
dc.date.issued2013-04-24en
dc.description.abstractComposting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the São Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. The phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo compost metagenomes compared with seven selected public metagenomes of biomass deconstruction environments, indicating the potential for different bacterial communities to provide alternative mechanisms for the same functional purposes. Our results indicate that biomass degradation in this composting process, including deconstruction of recalcitrant lignocellulose, is fully performed by bacterial enzymes, most likely by members of the Clostridiales and Actinomycetales orders.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0061928en
dc.identifier.eissn1932-6203en
dc.identifier.issue4en
dc.identifier.othere61928en
dc.identifier.pmid23637931en
dc.identifier.urihttp://hdl.handle.net/10919/85440en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleMetagenomic Analysis of a Tropical Composting Operation at the Sao Paulo Zoo Park Reveals Diversity of Biomass Degradation Functions and Organismsen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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