Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A + T rich

Simple item page
dc.contributor.authorBarbosa, Anne C.en
dc.contributor.authorXu, Zhengyaoen
dc.contributor.authorKarari, Kazhalen
dc.contributor.authorWilliams, Wendien
dc.contributor.authorHauf, Silkeen
dc.contributor.authorBrown, William R. A.en
dc.date.accessioned2022-09-02T13:58:10Zen
dc.date.available2022-09-02T13:58:10Zen
dc.date.issued2022-01-11en
dc.description.abstractWe have used chromosome engineering to replace native centromeric DNA with different test sequences at native centromeres in two different strains of the fission yeast Schizosaccharomyces pombe and have discovered that A + T rich DNA, whether synthetic or of bacterial origin, will function as a centromere in this species. Using genome size as a surrogate for the inverse of effective population size (N-e) we also show that the relative A + T content of centromeric DNA scales with N-e across 43 animal, fungal and yeast (Opisthokonta) species. This suggests that in most of these species the A + T content of the centromeric DNA is determined by a balance between selection and mutation. Combining the experimental results and the evolutionary analyses allows us to conclude that A + T rich DNA of almost any sequence will function as a centromere in most Opisthokonta species. The fact that many G/C to A/T substitutions are unlikely to be selected against may contribute to the rapid evolution of centromeric DNA. We also show that a neo-centromere sequence is not simply a weak version of native centromeric DNA and suggest that neo-centromeres require factors either for their propagation or establishment in addition to those required by native centromeres.en
dc.description.notesNottingham was supported by BBSRC [BB/K003356/1]; A.C.B. was funded by the Brazilian `NottinghamBirmingham' PhD scheme organised by CAPES, Brazil; K.K. was funded by the Kurdistan Regional Government, Human Capacity Development Program; S.H. and W.W. were supported by the National Institute of General Medical Sciences of the National Institutes of Health R35GM119723]. Funding for open access charge: University of Nottingham transformative agreement with OUP.en
dc.description.sponsorshipBBSRC [BB/K003356/1]; Brazilian 'NottinghamBirmingham' PhD scheme by CAPES, Brazil; Kurdistan Regional Government, Human Capacity Development Program; National Institute of General Medical Sciences of the National Institutes of Health [R35GM119723]; University of Nottingham; OUPen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/nar/gkab1219en
dc.identifier.eissn1362-4962en
dc.identifier.issn0305-1048en
dc.identifier.issue1en
dc.identifier.pmid34928384en
dc.identifier.urihttp://hdl.handle.net/10919/111692en
dc.identifier.volume50en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcenp-aen
dc.subjectartificial chromosomesen
dc.subjectheterochromatinen
dc.subjectevolutionen
dc.subjectratesen
dc.subjectcomplementationen
dc.subjectneocentromeresen
dc.subjectconstructionen
dc.subjectrequirementen
dc.subjectassociationen
dc.titleMutation and selection explain why many eukaryotic centromeric DNA sequences are often A + T richen
dc.title.serialNucleic Acids Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
gkab1219.pdf
Size:
3.94 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Scholarly Works, Biological Sciences
Scholarly Works, Fralin Life Sciences Institute