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A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

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dc.contributor.authorGilmore, Brian L.en
dc.contributor.authorWinton, Carly E.en
dc.contributor.authorDemmert, Andrew C.en
dc.contributor.authorTanner, Justin R.en
dc.contributor.authorBowman, Samen
dc.contributor.authorKarageorge, Vasileaen
dc.contributor.authorPatel, Kayaen
dc.contributor.authorSheng, Zhien
dc.contributor.authorKelly, Deborah F.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentBiomedical Engineering and Sciencesen
dc.date.accessioned2019-01-24T18:24:47Zen
dc.date.available2019-01-24T18:24:47Zen
dc.date.issued2015-09-23en
dc.description.abstractWe present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.en
dc.description.notesThis research is supported by funds from Virginia Tech, the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech, the Commonwealth Health Research Board [2080914], the Concern Foundation [303872], and NCI/NIH [R01CA193578] to D.F.K. C.W. is funded through the ICTAS Doctoral Scholar's program at Virginia Tech and the Medical Research Scholar's program at the Virginia Tech Carilion Research Institute.en
dc.description.sponsorshipVirginia Tech; Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech; Commonwealth Health Research Board [2080914]; Concern Foundation [303872]; NCI/NIH [R01CA193578]; ICTAS Doctoral Scholar's program at Virginia Tech; Medical Research Scholar's program at the Virginia Tech Carilion Research Instituteen
dc.format.extent8en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep14440en
dc.identifier.issn2045-2322en
dc.identifier.other14440en
dc.identifier.pmid26395823en
dc.identifier.urihttp://hdl.handle.net/10919/86881en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectrna-polymerase-iien
dc.subjectelectron-microscopyen
dc.subjectbreast-canceren
dc.subjectterminal domainen
dc.subjectaffinity gridsen
dc.subjectbrca1en
dc.subjecttranscriptionen
dc.subjectholoenzymeen
dc.subjectresistanceen
dc.titleA Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Diseaseen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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