Biomimetic Transparent Nanoplasmonic Meshes by Reverse-Nanoimprinting for Bio-Interfaced Spatiotemporal Multimodal SERS Bioanalysis

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dc.contributor.authorGarg, Adityaen
dc.contributor.authorMejia, Elieseren
dc.contributor.authorNam, Wonilen
dc.contributor.authorVikesland, Peter J.en
dc.contributor.authorZhou, Weien
dc.date.accessioned2023-05-08T18:03:53Zen
dc.date.available2023-05-08T18:03:53Zen
dc.date.issued2022-11en
dc.description.abstractMulticellular systems, such as microbial biofilms and cancerous tumors, feature complex biological activities coordinated by cellular interactions mediated via different signaling and regulatory pathways, which are intrinsically heterogeneous, dynamic, and adaptive. However, due to their invasiveness or their inability to interface with native cellular networks, standard bioanalysis methods do not allow in situ spatiotemporal biochemical monitoring of multicellular systems to capture holistic spatiotemporal pictures of systems-level biology. Here, a high-throughput reverse nanoimprint lithography approach is reported to create biomimetic transparent nanoplasmonic microporous mesh (BTNMM) devices with ultrathin flexible microporous structures for spatiotemporal multimodal surface-enhanced Raman spectroscopy (SERS) measurements at the bio-interface. It is demonstrated that BTNMMs, supporting uniform and ultrasensitive SERS hotspots, can simultaneously enable spatiotemporal multimodal SERS measurements for targeted pH sensing and non-targeted molecular detection to resolve the diffusion dynamics for pH, adenine, and Rhodamine 6G molecules in agarose gel. Moreover, it is demonstrated that BTNMMs can act as multifunctional bio-interfaced SERS sensors to conduct in situ spatiotemporal pH mapping and molecular profiling of Escherichia coli biofilms. It is envisioned that the ultrasensitive multimodal SERS capability, transport permeability, and biomechanical compatibility of the BTNMMs can open exciting avenues for bio-interfaced multifunctional sensing applications both in vitro and in vivo.en
dc.description.notesThis work was supported by AFOSR Young Investigator Award FA9550-18-1-0328 and by the US National Science Foundation grants OISE-1545756, CBET-2029911, CBET-2231807, and DMR-2139317. Laboratory and instrumentation support was provided by NanoEarth-a node of the NSF-supported NNCI (NSF award number #1542100). Additional support was provided by the Sustainable Nanotechnology Interdisciplinary Graduate Program (VTSuN IGEP), funded by Virginia Tech.en
dc.description.sponsorshipAFOSR Young Investigator Award [FA9550-18-1-0328]; US National Science Foundation [OISE-1545756, CBET-2029911, CBET-2231807, DMR-2139317]; NanoEarth-a node of the NSF-supported NNCI [1542100]; Sustainable Nanotechnology Interdisciplinary Graduate Program (VTSuN IGEP) - Virginia Techen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/smll.202204517en
dc.identifier.eissn1613-6829en
dc.identifier.issue45en
dc.identifier.other2204517en
dc.identifier.pmid36161480en
dc.identifier.urihttp://hdl.handle.net/10919/114973en
dc.identifier.volume18en
dc.language.isoenen
dc.publisherWiley-V C H Verlagen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectbacterial biofilmsen
dc.subjectbio-interfaced surface-enhanced Raman spectroscopy (SERS)en
dc.subjectmesh devicesen
dc.subjectmultimodal surface-enhanced Raman spectroscopy (SERS)en
dc.subjectnanoimprint lithographyen
dc.subjectsurface-enhanced Raman spectroscopy (SERS)en
dc.titleBiomimetic Transparent Nanoplasmonic Meshes by Reverse-Nanoimprinting for Bio-Interfaced Spatiotemporal Multimodal SERS Bioanalysisen
dc.title.serialSmallen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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