Diffusion-based Microfluidic PCR for "One-pot" Analysis of Cells

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dc.contributorVirginia Tech. School of Biomedical Engineering and Sciencesen
dc.contributorVirginia Tech. Department of Chemical Engineeringen
dc.contributor.authorMa, Saien
dc.contributor.authorLoufakis, Despina N.en
dc.contributor.authorCao, Zhenningen
dc.contributor.authorChang, Yiwenen
dc.contributor.authorAchenie, Luke E. K.en
dc.contributor.authorLu, Changen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessed2015-04-16en
dc.date.accessioned2015-04-20T22:22:11Zen
dc.date.available2015-04-20T22:22:11Zen
dc.date.issued2014-05-28en
dc.description.abstractGenetic analysis starting with cell samples often requires multi-step processing including cell lysis, DNA isolation/purification, and polymerase chain reaction (PCR) based assays. When conducted on a microfluidic platform, the compatibility among various steps often demands a complicated procedure and a complex device structure. Here we present a microfluidic device that permits a “one-pot” strategy for multi-step PCR analysis starting from cells. Taking advantage of the diffusivity difference, we replace the smaller molecules in the reaction chamber by diffusion while retaining DNA molecules inside. This simple scheme effectively removes reagents from the previous step to avoid interference and thus permits multi-step processing in the same reaction chamber. Our approach shows high efficiency for PCR and potential for a wide range of genetic analysis including assays based on single cells.en
dc.description.notesSupplementary information is included in a separate fileen
dc.description.sponsorshipVirginia Tech. Institute for Critical Technology and Applied Science. NanoBio Trusten
dc.description.sponsorshipNational Science Foundation (U.S.). Chemical, Bioengineering, Environmental and Transport Systems - Grant 1016547en
dc.description.sponsorshipNational Science Foundation (U.S.). Chemical, Bioengineering, Environmental and Transport Systems - Grant 0967069en
dc.description.sponsorshipNational Institutes of Health. National Cancer Institute - R21CA174577en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMa, S., Loufakis, D. N., Cao, Z., Chang, Y., Achenie, L. E. K., & Lu, C. (2014). Diffusion-based microfluidic PCR for "one-pot" analysis of cells. Lab on a Chip, 14(16), 2905-2909. doi: 10.1039/C4LC00498Aen
dc.identifier.doihttps://doi.org/10.1039/C4LC00498Aen
dc.identifier.issn1473-0197en
dc.identifier.urihttp://hdl.handle.net/10919/51726en
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2014/lc/c4lc00498aen
dc.language.isoenen
dc.publisherThe Royal Society of Chemistryen
dc.relation.ispartofseriesLab on a Chip Recent HOT Articlesen
dc.relation.urihttp://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=lc&themeid=9967dc51-4946-465e-a216-fa5407dc7f41en
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectGenetic analysisen
dc.subjectDNAen
dc.subjectPolymerase chain reaction assaysen
dc.titleDiffusion-based Microfluidic PCR for "One-pot" Analysis of Cellsen
dc.title.serialLab on a Chipen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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