T-DOpE probes reveal sensitivity of hippocampal oscillations to cannabinoids in behaving mice
| dc.contributor.author | Kim, Jongwoon | en |
| dc.contributor.author | Huang, Hengji | en |
| dc.contributor.author | Gilbert, Earl T. | en |
| dc.contributor.author | Kaiser C., Arndt | en |
| dc.contributor.author | English, Daniel Fine | en |
| dc.contributor.author | Jia, Xiaoting | en |
| dc.date.accessioned | 2024-08-19T14:52:07Z | en |
| dc.date.available | 2024-08-19T14:52:07Z | en |
| dc.date.issued | 2024-02-24 | en |
| dc.description.abstract | Understanding the neural basis of behavior requires monitoring and manipulating combinations of physiological elements and their interactions in behaving animals. We developed a thermal tapering process enabling fabrication of low-cost, flexible probes combining ultrafine features: dense electrodes, optical waveguides, and microfluidic channels. Furthermore, we developed a semi-automated backend connection allowing scalable assembly. We demonstrate T-DOpE (Tapered Drug delivery, Optical stimulation, and Electrophysiology) probes achieve in single neuron-scale devices (1) highfidelity electrophysiological recording (2) focal drug delivery and (3) optical stimulation. The device tip can beminiaturized (as small as 50 μm) tominimize tissue damage while the ~20 times larger backend allows for industrial-scale connectorization. T-DOpE probes implanted in mouse hippocampus revealed canonical neuronal activity at the level of local field potentials (LFP) and neural spiking. Taking advantage of the triple-functionality of these probes, we monitored LFP while manipulating cannabinoid receptors (CB1R; microfluidic agonist delivery) and CA1 neuronal activity (optogenetics). Focal infusion of CB1R agonist downregulated theta and sharp wave-ripple oscillations (SPWRs). Furthermore, we found that CB1R activation reduces sharp wave-ripples by impairing the innate SPW-R-generating ability of the CA1 circuit. | en |
| dc.description.sponsorship | X.J. gratefully acknowledges funding support from the National Institute of Health (R01NS123069, R21EY033080, R56AG077720) and National Science Foundation (ECCS- 1847436). DFE gratefully acknowledges funding support from The Simons Foundation and The Whitehall Foundation. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1038/s41467-024-46021-4 | en |
| dc.identifier.uri | https://hdl.handle.net/10919/120958 | en |
| dc.identifier.volume | 15 | en |
| dc.language.iso | en | en |
| dc.publisher | Nature Research | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.title | T-DOpE probes reveal sensitivity of hippocampal oscillations to cannabinoids in behaving mice | en |
| dc.title.serial | Nature Communications | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |