In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds

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dc.contributor.authorAlbanna, Mohammeden
dc.contributor.authorBinder, Kyle W.en
dc.contributor.authorMurphy, Sean V.en
dc.contributor.authorKim, Jaehyunen
dc.contributor.authorQasem, Shadi A.en
dc.contributor.authorZhao, Weixinen
dc.contributor.authorTan, Joshen
dc.contributor.authorEl-Amin, Idris B.en
dc.contributor.authorDice, Dennis D.en
dc.contributor.authorMarco, Julieen
dc.contributor.authorGreen, Jasonen
dc.contributor.authorXu, Taoen
dc.contributor.authorSkardal, Aleksanderen
dc.contributor.authorHolmes, James H.en
dc.contributor.authorJackson, John D.en
dc.contributor.authorAtala, Anthonyen
dc.contributor.authorYoo, James J.en
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2019-07-23T17:51:51Zen
dc.date.available2019-07-23T17:51:51Zen
dc.date.issued2019-02-21en
dc.description.abstractThe early treatment and rapid closure of acute or chronic wounds is essential for normal healing and prevention of hypertrophic scarring. The use of split thickness autografts is often limited by the availability of a suitable area of healthy donor skin to harvest. Cellular and non-cellular biological skin-equivalents are commonly used as an alternative treatment option for these patients, however these treatments usually involve multiple surgical procedures and associated with high costs of production and repeated wound treatment. Here we describe a novel design and a proof-of-concept validation of a mobile skin bioprinting system that provides rapid on-site management of extensive wounds. Integrated imaging technology facilitated the precise delivery of either autologous or allogeneic dermal fibroblasts and epidermal keratinocytes directly into an injured area, replicating the layered skin structure. Excisional wounds bioprinted with layered autologous dermal fibroblasts and epidermal keratinocytes in a hydrogel carrier showed rapid wound closure, reduced contraction and accelerated re-epithelialization. These regenerated tissues had a dermal structure and composition similar to healthy skin, with extensive collagen deposition arranged in large, organized fibers, extensive mature vascular formation and proliferating keratinocytes.en
dc.description.notesWe would like to thank the WFIRM surgical core facility, in particular Cara Clouse, Tammy Cockerham, Tiffany Bledsoe, Adam Wilson and Sandy Sink. We also thank Paul Robertson (Next Medical Design) and Kevin Rackers, the contract developers of the printer. This research was funded by the Telemedicine and Advanced Technology Research Center and the Armed Forces Institute for Regenerative Medicine.en
dc.description.sponsorshipTelemedicine and Advanced Technology Research Centeren
dc.description.sponsorshipArmed Forces Institute for Regenerative Medicineen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-018-38366-wen
dc.identifier.issn2045-2322en
dc.identifier.other1856en
dc.identifier.pmid30755653en
dc.identifier.urihttp://hdl.handle.net/10919/91930en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectpressure ulcersen
dc.subjectkeratinocytesen
dc.subjectburnsen
dc.subjectfibroblastsen
dc.subjectfabricationen
dc.subjectmanagementen
dc.subjectbiobraneen
dc.subjectmatrixen
dc.titleIn Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Woundsen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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