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Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure

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dc.contributor.authorAgoglia, Abigail E.en
dc.contributor.authorZhu, ManHuaen
dc.contributor.authorYing, Roseen
dc.contributor.authorSidhu, Harpreeten
dc.contributor.authorNatividad, Luis A.en
dc.contributor.authorWolfe, Sarah A.en
dc.contributor.authorBuczynski, Matthew W.en
dc.contributor.authorContet, Candiceen
dc.contributor.authorParsons, Loren H.en
dc.contributor.authorRoberto, Marisaen
dc.contributor.authorHerman, Melissa A.en
dc.contributor.departmentSchool of Neuroscienceen
dc.date.accessioned2020-05-13T19:15:11Zen
dc.date.available2020-05-13T19:15:11Zen
dc.date.issued2020-03en
dc.description.abstractThe lateral amygdala (LA) serves as the point of entry for sensory information within the amygdala complex, a structure that plays a critical role in emotional processes and has been implicated in alcohol use disorders. Within the amygdala, the corticotropin-releasing factor (CRF) system has been shown to mediate some of the effects of both stress and ethanol, but the effects of ethanol on specific CRF1 receptor circuits in the amygdala have not been fully established. We used male CRF1:GFP reporter mice to characterize CRF1-expressing (CRF1(+)) and nonexpressing (CRF1(-)) LA neurons and investigate the effects of acute and chronic ethanol exposure on these populations. The CRF1(+) population was found to be composed predominantly of glutamatergic projection neurons with a minority subpopulation of interneurons. CRF1(+) neurons exhibited a tonic conductance that was insensitive to acute ethanol. CRF1(-) neurons did not display a basal tonic conductance, but the application of acute ethanol induced a delta GABA(A) receptor subunit-dependent tonic conductance and enhanced phasic GABA release onto these cells. Chronic ethanol increased CRF1(+) neuronal excitability but did not significantly alter phasic or tonic GABA signaling in either CRF1(+) or CRF1(-) cells. Chronic ethanol and withdrawal also did not alter basal extracellular GABA or glutamate transmitter levels in the LA/BLA and did not alter the sensitivity of GABA or glutamate to acute ethanol-induced increases in transmitter release. Together, these results provide the first characterization of the CRF1(+) population of LA neurons and suggest mechanisms for differential acute ethanol sensitivity within this region.en
dc.description.notesThis work was supported by the Bowles Center for Alcohol Studies; and National Institutes of Health Grants T32-AA-007573 (A.E.A.), T32-NS-007431 (M.Z.), AA-023002 (M.A.H.), AA-011605 (M.A.H.), AA-015566 (M.R.), AA-021491 (M.R.), AA-006420 (C.C., M.R.), T32-AA-007456 (S.A.W.), AA-026685 (C.C.), and AA-024952 (H.S.).en
dc.description.sponsorshipBowles Center for Alcohol Studies; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [T32-AA-007573, T32-NS-007431, AA-023002, AA-011605, AA-015566, AA-021491, AA-006420, T32-AA-007456, AA-026685, AA-024952]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1523/ENEURO.0420-19.2020en
dc.identifier.eissn2373-2822en
dc.identifier.issue2en
dc.identifier.other0420-19.2020en
dc.identifier.pmid32041742en
dc.identifier.urihttp://hdl.handle.net/10919/98248en
dc.identifier.volume7en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectalcoholen
dc.subjectbasolateral amygdalaen
dc.subjectCRFen
dc.subjectCRF1 receptoren
dc.subjectGABAen
dc.subjectlateral amygdalaen
dc.titleCorticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposureen
dc.title.serialeNeuroen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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