Optical sectioning with a low-coherence phase-shifting digital holographic microscope
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Date
2011-03-01
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Publisher
Optical Society of America
Abstract
The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3 mu m and axial resolution of 10 mu m through a thickness of 300 mu m onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment. (C) 2010 Optical Society of America
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Keywords
Scanning holography, 3-dimensional microscopy, Interference holography, Image-reconstruction, Tomography
Citation
Yu-Chih Lin, Chau-Jern Cheng, and Ting-Chung Poon, "Optical sectioning with a low-coherence phase-shifting digital holographic microscope," Appl. Opt. 50, B25-B30 (2011); doi: 10.1364/ao.50.000b25