Flatbed Scanning as a Novel Approach for Examining Lipid Distribution in Fish: Comparison with MRI and Traditional Chemical Methods

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dc.contributor.authorWille, K.en
dc.contributor.authorJain, R.en
dc.contributor.authorMcLean, E.en
dc.contributor.authorGoddard, J. S.en
dc.contributor.authorKaplan, J.en
dc.contributor.authorLeven, H.en
dc.contributor.editorSmith, Stephen Allenen
dc.date.accessioned2019-06-25T16:55:10Zen
dc.date.available2019-06-25T16:55:10Zen
dc.date.issued2004-06-01en
dc.description.abstractDepending upon species, the concentration and distribution of lipid in fish flesh impacts both processing requirements and eating quality. Dispersal of lipid within fish muscle may be manipulated by diet, feeding strategy, and through selective breeding. Several methods are currently used to examine lipid deposition in fish, but these are either arduous, costly, or reliant upon noxious chemicals. The need exists for a rapid, inexpensive, and safe method for examining lipid distribution in fish flesh. A technique that satisfies the preceding criteria was developed. Fish cutlets were stained with oil red O, scanned, and the images saved in 600 dpi *.tiff format. Oil red O was employed to differentiate muscle tissue from lipid. Cutlets were examined using computer-assisted image analysis and lipid presence in each cutlet recorded in percent terms. The results were compared to data generated from the same cutlets using Magnetic Resonance Imaging to separate muscle from lipid. No differences were detected between methods with regards to lipid distribution, which followed an anterior to posterior decline in the body. Lipid dispersal did not differ with gender. Estimates of total lipid in scanned images were identical to those recorded using chemical analysis.en
dc.format.extent17 pagesen
dc.format.extent2.13 MBen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationWille, K., Jain, R., McLean, E., Goddard, J.S., Kaplan, E.J. and Leven, H., 2004. Flatbed Scanning as a Novel Approach for Examining Lipid Distribution in Fish: Comparison with MRI and Traditional Chemical Methods. International Journal of Recirculating Aquaculture, 5. DOI: http://doi.org/10.21061/ijra.v5i1.1388en
dc.identifier.doihttps://doi.org/10.21061/ijra.v5i1.1388en
dc.identifier.eissn2572-9160en
dc.identifier.issn1547-917Xen
dc.identifier.issue1en
dc.identifier.urihttp://hdl.handle.net/10919/90598en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherCommercial Fish and Shellfish Technologies Program, Virginia Techen
dc.rightsIn Copyrighten
dc.rights.holderCommercial Fish and Shellfish Technologies Program, Virginia Techen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectFat Depositionen
dc.subjectRecirculating Aquacultureen
dc.titleFlatbed Scanning as a Novel Approach for Examining Lipid Distribution in Fish: Comparison with MRI and Traditional Chemical Methodsen
dc.title.serialInternational Journal of Recirculating Aquacultureen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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