Browsing by Author "Malhotra, B. D."
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- Electrochemical Urea Biosensor Based on Sol-gel Derived Nanostructured Cerium OxideAnsari, Anees A.; Ali, Md. Azahar; Malhotra, B. D. (World Scientific, 2012-04-18)Urease (Urs) and glutamate dehydrogenase (GLDH) have been co-immobilized onto a nanostructured–cerium oxide (Nano-CeO2) film deposited onto a indium-tin-oxide (ITO) coated glass substrate by dip-coating via sol-gel process for urea detection. This nanostructured film has characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), Scanning electron microscope (SEM) and electrochemical techniques, respectively. The particle size of the Nano-CeO2 film has been found to be 23 nm. Electrochemcial response (CV) studies show that Ur-GLDH/Nano-CeO2/ITO bioelectrode is found to be sensitive in the 10–80 mg/dL urea concentration range and can detect urea concentration upto 0.1 mg/dL level. The value of Michaelis–Menten constant (Km) estimated using Lineweaver–Burke plot found as 6.09 mg/dL indicates enhancement in the affinity and/or activity of enzyme attached to their nanobiocomposite. This bioelectrode retained 95% of enzyme activity after 6 months at 4°C.
- Fabrication of nanocrystalline CdS electrode via chemical bath deposition technique for application to cholesterol sensorDhyani, Hemant; Srivastava, Saurabh; Ali, Md. Azahar; Malhotra, B. D.; Sen, Prasenjit (IOP, 2012-04-18)A nanocystalline CdS electrode has been fabricated by chemical bath deposition (CBD) technique onto hydrolyzed indium tin oxide (ITO) coated glass substrate at 78°C for the immobilization of cholesterol oxidase (ChOx). The prepared Nano-CdS based electrode has been characterized using UV-visible, X-ray diffraction (XRD), Fourier transform-infrared (FTIR) and scanning electron microscopy (SEM). The ChOx/Nano-CdS/ITO bioelectrode shows the detection range of cholesterol from 50 to 400 mg/dl with improved sensitivity of 1.35 μA/mgdl−1/cm2, low detection limit (6.1 mg/dl) and low Km (0.45mM) value indicating strong enzyme (cholesterol oxidase)-matrix (CdS) affinity.