Fabrication of nanocrystalline CdS electrode via chemical bath deposition technique for application to cholesterol sensor

A nanocystalline CdS electrode has been fabricated by chemical bath deposition (CBD) technique onto hydrolyzed indium tin oxide (ITO) coated glass substrate at 78°C for the immobilization of cholesterol oxidase (ChOx). The prepared Nano-CdS based electrode has been characterized using UV-visible, X-ray diffraction (XRD), Fourier transform-infrared (FTIR) and scanning electron microscopy (SEM). The ChOx/Nano-CdS/ITO bioelectrode shows the detection range of cholesterol from 50 to 400 mg/dl with improved sensitivity of 1.35 μA/mgdl−1/cm2, low detection limit (6.1 mg/dl) and low Km (0.45mM) value indicating strong enzyme (cholesterol oxidase)-matrix (CdS) affinity.