VTechWorks Repository :: Browsing by Author "Nilsen, Erik T."

Browsing by Author "Nilsen, Erik T."

Now showing 1 - 20 of 53

The acclimation ability of the shale barren endemic Eriogonum alleni to light and heat
Braunschweig, Suzanne Hill (Virginia Tech, 1993-09-04)
Shale barrens are unique habitats located throughout the southern Appalachians. They are characterized by a south or south west aspect, a steep slope, and an exposed rocky surface (Platt, 1951). They have a high total irradiance and can experience temperatures higher than the surrounding deciduous forest. A variety of plant species, several of which are rare or endangered, are endemic to the shale barren habitat. One reason proposed fc)r their endemism is that the plants are obligate heliophytes (Keener, 1983). The purpose of this dissertation is tel examine the acclimation ability of the shale barren endemic Eriogonum alIeni to shade and high temperature.
An analysis of correlated curve trend experiments in Eucalyptus grandis
Bredenkamp, Brian Victor (Virginia Polytechnic Institute and State University, 1988)
Correlated curve trend (C.C.T.) experiments in Eucalyptus grandis on the Zululand coast of South Africa were analyzed. Growth parameters were described as functions of age using Schnute’s generalized growth function and parameter estimates were described as functions of stand density. Growth attributes were used as moments of a probability density function to describe a diameter distribution model for the species. Time trends in the relationships between growth parameters and stand density were scrutinized with multiple comparisons of paired means. It was shown that diameter growth in lower size classes ceases under conditions of extreme suppression while growth continues unabated in the larger size classes, resulting in greater dispersion in diameter. Competition mortality was to a large extent confined to the lower size classes and severe mortality results in an apparent increase in mean diameter which precludes use of growth functions which impose an asymptote. Allometric growth was investigated on two different sites and growth trends were shown to be anamorphic between sites. This permits a ratio approach to the estimation of growth and yield on one site based on experimental evidence from another. Thinning effects in terms of diameter and height changes were estimated from simulated thinnings using data from unthinned stands while the results of long-term thinning studies were compared in terms of cumulative volume yields. The age at which mean annual increment culminates was determined and a model for the estimation of m.a.i. as a function of age and stand density was constructed. A critical examination of spacing indices revealed that the slopes thereof were much steeper than those for many other species. The better-known indices of Reineke and Yoda were found to be dependent on age.
Analysis of intraspecific and interspecific interactions between the invasive exotic tree-of-heaven (Ailanthus altissima (Miller) Swingle) and the native black locust (Robinia pseudoacacia L.)
Call, Lara J. (Virginia Tech, 2002-05-09)
Invasive exotic plants can persist and successfully spread within ecosystems and negatively affect the recruitment of native species. The exotic invasive Ailanthus altissima and the native Robinia pseudoacacia are frequently found in disturbed sites and exhibit similar growth and reproductive characteristics, yet each has distinct functional roles such as allelopathy and nitrogen fixation, respectively. 1) A four-month full additive series in the greenhouse and 2) spatial point pattern analysis of trees in a silvicultural experiment were used to analyze the intraspecific and interspecific interference between these two species. In the greenhouse experiment, total biomass responses per plant for both species were significantly affected by interspecific but not by intraspecific interference (p <0.05). Competition indices such as Relative Yield Total and Relative Crowding Coefficient suggested that A. altissima was the better competitor in mixed plantings. Ailanthus altissima consistently produced a larger above ground and below ground relative yield while R. pseudoacacia generated a larger aboveground relative yield in high density mixed species pots. However, R. pseudoacacia exhibited more variation for multiple biomass traits, occasionally giving it an above ground advantage in some mixed species pots. Analysis of spatial point patterns in the field with Ripley's K indicated that the two species were positively associated with each other along highly disturbed skid trails in the majority of the field sites. Locally, increased disturbances could lead to more opportunities for A. altissima to invade, negatively interact with R. pseudoacacia (as was evident in the greenhouse study), and become established in place of native species.
An analysis of palustrine forested wetland compensation effectiveness in Virginia
Atkinson, Robert B. (Virginia Tech, 1991-09-03)
Plans to construct a wetland to replace wetland losses has become a common feature of permit requests. The purpose of this project is to suggest a methodology for quantifying the effectiveness of palustrine forested wetland construction in Virginia. Wetlands constructed by ~ne Virginia Department of Transportation and the U.S. Army Corps of Engineers were surveyed and Wagner Road constructed wetland in Petersburg, Virginia was selected as the primary study site. Chapter One of the present study suggests a method for early assessment of revegetation success utilizing weighted averages of colonizing vegetation. An adjacent reference site was chosen that was in close proximity to the constructed site and was used for comparison. Results from the Wagner Road site and the reference wetland indicated that colonizing vegetation weighted averages provide a more sensitive measure of revegetation success than the methods described in the federal wetland delineation manual.
Biotic Interaction of Invasive, Early-Succession Trees and Their Effects on Community Diversity: a Multi-Scale Study Using the Exotic Invasive Ailanthus altissima and the Native Robinia pseudoacacia in the Mid-Appalachian Forest of Eastern United States
Bao, Zhe (Virginia Tech, 2015-04-28)
Invasive plants can displace native species, deteriorate native forest, and change plant communities and ecosystem functions. Native plant populations are fundamentally impacted by invasive species because of the interactions between invasive species and native plants. This study focuses on understanding the extent, mechanisms and consequences of interaction between a non-indigenous invader Ailanthus altissima and its functionally similar native species Robinia pseudoacacia in the Mid-Appalachian region, from an individual scale to a regional scale. These two subject species are common and coexist in early-successional eastern deciduous forest. The interactions between these two common species are important to community structure and canopy tree regeneration. To address the type and extent of interactions of these two species, a greenhouse experiment utilizing various species proportions, nutrient levels and seed sources was performed. In addition, a common-garden experiment with various species densities and proportions over three consecutive growing seasons was performed in a more natural condition than that of the greenhouse experiment. We found at the seedling stage, the dominant interaction was competition, and R. pseudoacacia was the winner both above- and belowground. The allelopathic compounds of A. altissima may have inhibited nodulation of R. pseudoacacia. Ailanthus altissima seedlings from its native region had slightly stronger competitive abilities compared with the seedlings from its invaded range. In the common garden experiment, R. pseudoacacia plants grew quicker than A. altissima, but A. altissima inhibited the growth of R. pseudoacacia by interspecific competition. The negative impact of A. altissima on R. pseudoacacia became larger as time progressed. To assess the community-level consequences of the two species, we conducted a forest mapping and a complete target-tree-based forest survey, and analyzed regional-scale data from the Forest Inventory Analysis Data Base. The two target species were significantly associated with themselves and with each other. Community species composition and diversity were significantly different across sites. A negative impact of both species on the understory community diversity and tree regeneration at the neighborhood scale was detected; while at a regional level, tree diversity in the FIA plots with either A. altissima or R. pseudoacacia was higher than the reference plots.
The combined effects of fertilization and relative water limitation on tissue water relations, hydraulic parameters and shallow root distribution in loblolly pine (Pinus taeda L.)
Russell, Edward Morgan (Virginia Tech, 2019-08-27)
One goal of this research was to characterize shoot tissue-level responses in loblolly pine to soil moisture limitation in combination with fertilization as well as to more severe soil moisture limitation. We found that neither fertilization alone, nor fertilization in combination with soil moisture limitation resulted in changes to shoot tissue water relations parameters classically characterized in drought response studies. More severe water limitation was necessary to elicit responses, and those responses had not been fully described previously. The more severe water limitation resulted in increased capacitance beyond turgor loss, increased relative water content at turgor loss, a more negative turgor loss point, an increased bulk modulus of elasticity, more negative osmotic potential at 100% relative water content, and an increased apoplastic water fraction. As there were indications of reduced water use and moisture stress in the absence of shoot level responses under less severe drought, such parameters are insufficient alone to characterize moisture stress in fertilized and in less severely water limited loblolly trees. Additionally, we sought a morphological or physiological explanation for the reduced transpiration and increased water use efficiency reported for fertilized trees in the Virginia Piedmont. Our characterizations of the responses of root distribution and hydraulics to limited soil moisture here complement existing research, which demonstrated changes to root distribution and hydraulics in response to fertilization. The responses we discovered in fertilized trees that accompanied reduced transpiration and increased water use efficiency that differed from responses to reduced soil moisture alone were primarily large decreases to shallow root presence. We found this to be readily quantified using measures of root length density. Decreases to whole-tree hydraulic conductivity were also shown to occur with fertilization and were shown not to occur in shoot tissue, suggesting limitation via rhizosphere or root xylem conductance. Our results support the supposition that fertilization narrows hydraulic safety margins and potentially predisposes loblolly trees to moisture stress, particularly prolonged, severe water limitation following fertilization. Finally, we tested the validity of throughfall exclusion for simulating reduced rainfall using a greenhouse 'split-pot' study, which applied spatially fixed heterogeneous soil moisture to young, well-watered loblolly pines. The 'split-pot' experiments demonstrated that spatially fixed soil moisture heterogeneity does not confound drought effects; needle area specific transpiration was not decreased, nor was water use efficiency increased. This supports the validity of inferences taken from drought simulation experiments with loblolly pine where throughfall exclusion troughs reduce soil moisture content in a consistent, spatially heterogeneous manner.
Comparative physiology of radish populations with differential sensitivity to O₃ and SO₂
Gillespie, Chris Todd (Virginia Polytechnic Institute and State University, 1989)
Radish plants (Raphanus sativus L. cv Cherry Belle) were exposed to 0.10 μl l⁻¹ ozone (O₃) or 0.50 μl l⁻¹ sulfur dioxide (SO2) for 4 h d⁻¹, 3 d wk⁻¹ for 3 weeks. From these fumigated plants, individuals were selected that were resistant or sensitive to these pollutants. The selected plants were used as parental material in a breeding program to produce lines differing in resistance to O₃ and SO₂. Non-selected (NS) plants from the original populations served as controls. F₁ populations were raised and exposed to O₃ or SO₂ with the same fumigation regime used for the parents. The plants were harvested 30 days after emergence and dry weights were determined. Plants selected for O₃ resistance (O3R) weighed significantly more than either plants selected for sensitivity to O₃ (O₃S) or NS plants when exposed to either O₃ or SO₂. The hypocotyl was most affected by pollutant exposure, leading to reduced root/shoot ratios. Plants selected for resistance or sensitivity to SO₂ generally had biomass production similar to that of NS plants. Growth analysis at early stages of growth indicated that both O₃R and O₃S plants had less growth under O₃ fumigated conditions; however, by maturity O₃R plants had similar amounts of growth under fumigated or non-fumigated conditions. Ozone fumigations tended to decrease free sugar concentrations in leaves at early stages of growth in both O₃R and O₃S plants, and caused some accumulations of carbohydrates during late stages of growth in O₃S plants. Allocation of Mc was significantly lower to hypocotyls and roots of O₃ fumigated O₃S plants. Allocation to hypocotyls of O₃R plants was not affected, although both O₃R and O₃S plant groups had lower photosynthetic rates due to O₃ fumigation. Ozone did not significantly affect chlorophyll concentrations in leaves of either sensitive or resistant plants, nor was the time of new leaf production affected by fumigation. These experiments demonstrated the potential of O₃ to influence the composition of sensitive plant populations. However, SO₂ was a much less powerful influence on the composition of these populations.
Comparative water relations of phreatophytes in the sonoran desert of california
Nilsen, Erik T.; Sharifi, M. Rasoul; Rundel, Philip W. (Ecological Society of America, 1984)
The seasonal and diurnal water relations were compared among six desert phreatophytes, two evergreen shrubs, and one deciduous shrub. All species were located in one wash woodland in the Sonoran Desert of southern California. There are several mechanisms by which these Phaenicia have adapted to the desert environment. One group of winter-deciduous phreatophytes (Olneya tesota, Prosopis glandulosa, and Acacia greggii) experienced summer midday leaf water potentials below -4.0 MPa. These phreatophytes had a series of physiological mechanisms for tolerating summer water stress, including seasonal and diurnal osmotic adjustment and the maintenance of high leaf conductance at low leaf water potential. Osmotic adjustment of these three phreatophytes was similar to or greater than that of two evergreen species (Larrea tridentata and Simmondsia chinensis). Dalea spinosa, a stem-photosynthetic phreatophyte, avoided water stress by maintaining a very small leaf area. The summer-deciduous phreatophytes (Hyptis emoryi, and Chilopsis linearis) demonstrated mechanisms of drought avoidance such as change in leaf biomass and low summer leaf conductance. Little osmotic adjustment occurred in the summer-deciduous phreatophytes. The phreatophytic species studied in this investigation have evolved adaptations to water stress that are similar to those of deciduous and evergreen shrubs of the Sonoran Desert. Desert phreatophytes are a complex group of species with varied adaptive mechanisms to tolerate or avoid drought and should not be considered simply as a group of species that avoid desert water stress by utilizing deep ground water unavailable to other desert species of drought tolerance and avoidance.
Cool Temperature Effects on Productivity and Photosynthesis of Two Biomass Fuel Species: Switchgrass (Panicum virgatum) and Miscanthus (Miscanthus x giganteus)
Mitchell, Jackson Lee Bean (Virginia Tech, 2013-01-14)
The world\'s highest yielding crops are C4 plants due to their higher water use efficiency, nitrogen use efficiency, and productivity compared with C3 plants. With an increasing demand for renewable resources as a result of the decreasing global supplies of fossil fuels, we need to improve our understanding of the limitations of biomass fuel feedstock to improve yields and better satisfy energy requirements. The ability to attain the goal feedstock production in the US is limited by available arable land and cool temperatures. This study investigates the effects of cool temperatures on the productivity and photosynthesis of the two species with the highest potential for feedstock production in the US: switchgrass (Panicum virgatum) cv. Alamo and miscanthus (Miscanthus × giganteus). At 14/12"C and a 14/10 hour light/dark photoperiod, switchgrass showed lower productivity and light saturated photosynthetic rates (Amax=10.3 "mol m-2s-1) compared with 28/25"C and the same photoperiod (Amax=18.8 "mol m-2s-1). Miscanthus has demonstrated cold tolerance in previous studies, and here showed no significant decrease in the productivity or photosynthetic rates in cool, compared with warm, growing conditions (Amax=8.2 "mol m-2s-1 and 7.0 "mol m-2s-1 for warm and cool conditions, respectively). Also, this study examines the potential limitations of C4 photosynthesis by the enzyme pyruvate phosphate dikinase (PPDK) under the same cool conditions, transgenic switchgrass cv. Alamo were created with the insertion of the miscanthus PPDK gene. Productivity and photosynthetic responses of the transgenic plants were evaluated in cool and warm growth temperatures. Of the two transgenic events tested here, line S(1) displayed cold tolerance, as seen in no loss of both carboxylation efficiency and the ratio of CO2 assimilation to electron transport (Asat/Jmax). These results indicate that PPDK may pose a significant limitation to C4 photosynthesis in cool conditions and there is a possibility that cold season photosynthesis of switchgrass cv. Alamo could be improved.
Diurnal and seasonal water relations of the desert phreatophyte prosopis-glandulosa (honey mesquite) in the sonoran desert of california
Nilsen, Erik T.; Sharifi, M. Rasoul; Rundel, Philip W.; Jarrell, Wesley M.; Virginia, Ross A. (Ecological Society of America, 1983)
Diurnal and Seasonal water relations were monitored in a population of Prosopis glandulosa var. torreyana in the Sonoran Desert of southern California. Prosopis glandulosa at this research site acquired its water from a ground water source 4-6 m deep. Measurements of diurnal and seasonal cycles of aboveground environmental conditions, soil moisture, and soil water potential (to 6 m depth) were taken to ascertain environmental water availability and water stress. Leaf water potential, leaf conductance, leaf transpiration, relative saturation deficit of leaves, osmotic potential, and turgor potential were measured to evaluate plant adaptations to environmental water stress. Soil water potential was low (-4.0 to -5.0 MPa) in surface soil in relation to deep soil (-0.2) MPa). This difference was due to high surface soil salinity and low surface water content. the climatic conditions at the research site produced extreme water stress conditions in summer months when temperatures reached 50-C, vapor pressure deficit (VPD) reached 8 kPa, and surface soil water potential was below -4.5 MPa. Although considerable plant water stress developed in these trees (midday leaf water potential -4.8 MPa), osmotic adjustment occurred and turgor was maintained on a diurnal and seasonal cycle. Prosopis glandulosa has adapted to avoid water stress by utilizing deep ground water, but this phreatophyte has also evolved physiological adaptations, such as osmotic adjustment and seasonally changing stomatal sensitivity to VPD, which result in greater tolerance of water stress.
Diversity, Invasibility, and Stability of Appalachian Forests across an Experimental Disturbance Gradient
Belote, R. T. (Virginia Tech, 2008-09-05)
For this dissertation, I measured how plant communities in Appalachian forests responded to disturbances caused by forest management activities. I had two primary objectives including (1) testing theories of biological diversity and invasions by nonnative species; and (2) providing empirical data that will help guide the sustainable use of forest resources. This work is part of the Southern Appalachian Silviculture and Biodiversity (SASAB) experiment that was established in the early 1990s to investigate ecosystem responses to a gradient of timber harvesting disturbances. Ranging from undisturbed controls to silvicultural clearcuts, the disturbance gradient is replicated at sites located throughout the Appalachian mountains of Virginia and West Virginia. The plant community was sampled across a wide range of spatial scales (2 hectares to 1 m2) using a nested sampling design and was also sampled at three times including pre-disturbance, one year post-disturbance, and ten year post-disturbance. For one element of the study I tested modern theories of biological invasions and investigated how the forest disturbance gradient interacted with species diversity to influence invasion by nonnative species (Chapter 2). Contrary to popular ecological theories of biotic resistance, the most diverse sites tended to be more easily invaded following intense canopy disturbance. Interestingly, none of the sites in this study were dominated by invasive plants, which led me to ask whether dominant tree species in forests provided resistance to nonnative plant establishment and growth through the quality of the litter they produce. I also asked how might animals that are known to alter litter layers interact with dominant tree species to influence plant invasions. Therefore, I conducted an experiment investigating how changes in litter from dominant tree species and invasions by nonnative earthworms might influence invasibility of forests using forest floor mesocosms (Chapter 3). I found that plant invasion was inhibited by native oak litter even when earthworms were present, suggesting that oak forests may resist plant invasions via oak tree litter. In contrast, plant invasion was greater under invasive tree litter and earthworm activity tended to facilitate invasive plant success only under invasive tree litter. I was also interested in understanding how disturbance might alter relationships between local and regional diversity. The long-term data of the forest disturbance experiment allowed me to investigate how local species richness is mediated by regional species richness after disturbance and during forest community development (Chapter 4). Local richness depended strongly on regional richness only after disturbance via colonization of species, but this relationship changed during forest aggradation. These results suggest that regional species pools are important to maintain local diversity following disturbance, but that local interactions (through canopy closure of dominant trees) exert control over species diversity during community reorganization. Lastly, I tested current theories on how diversity influences compositional stability after disturbance (Chapter 5). Disturbance consistently resulted in decreased compositional stability, but diversity was associated with stability in complex ways, which depended on how stability was measured and at what scale. Species-rich areas were in some instances less stable; in other instances areas with intermediate levels of diversity were more stable. These results suggest that disturbance causes shifts in species composition via colonization, but the ways in which diversity of sites influences compositional stability is complex and depends on methods used and the scales of observation. Taken together, these results suggest that disturbance influences invasibility, species saturation, and compositional stability of ecological communities. These properties change immediately following disturbance, and during forest development and canopy closure. Data from this project were useful in testing existing theories of community ecology, and may ultimately prove useful for forest managers as they decide how to protect biodiversity while planning for other uses of forest resources. Overall, these results suggest that colonization of species is the primary process driving plant community patterns in Appalachian forests following disturbance.
The ecology and population biology of two litter decomposing basidiomycetes
Murphy, John F. (Virginia Tech, 1992)
Four 286m2 plots on alternate sides of the spur ridges on Brush Mt. were established and their plant communities characterized. Agaric and Bolete basidiomycetes were sampled for two years. Fifty species were recorded on the plots. Phenologically, decomposer species were highly dependent upon rainfall events, whereas mycorrhizal species were more seasonal. Two species emerged as dominant litter decomposers. Marasmiellus praeacutus (Ellis) Halling is dominant on southwest facing slopes and occurs on a wide variety of coniferous and hardwood detritus. Collybia subnuda (Ellis ex. Peck) Gilliam is dominant on northeast facing slopes, and occurs on hardwood leaves and small woody detritus. The population structure of both of these species was investigated using tests of somatic incompatibility. Genets of both species are able to persist for more than one year. The observed minimum population density is 0.071 - 0.121 genets/m² for M. praeacutus and 0.039 - 0.093 genets/m² for g. subnuda. Mating tests indicate that M. praeacutus is heterothallic and tetrapolar, and that C. subnuda is heterothallic and bipolar. Preliminary crosses between monokaryotic tester sets indicate a surprisingly low number of mating alleles in both species. Decomposition studies suggest that while the restricted distribution of ~. subnuda to the northeast slopes may be affected by substrate specificity, the restriction of M. praeacutus to the southwest slopes is due to other factors.
Ecology of Root-Feeding Insect Assemblages in Fire-Manipulated Longleaf Pine-Wiregrass Ecosystems
Dittler, Matthew Jason (Virginia Tech, 2013-05-23)
Root-feeding insects can have top-down influence on vegetative composition and ecosystem processes; however, they may respond to bottom-up factors such as soil resources, site productivity, and disturbance. My research addressed the following questions: (1) Do disturbance (fire), vegetative composition, soil resources, and fine root standing mass influence the structure of root-feeding insect assemblages? (2) What types of roots do root-feeding insects eat, and do they forage selectively? (3) Do root-feeding insects influence fine root productivity? To address these questions, I studied root-feeding insect assemblages in longleaf pine wiregrass (Pinus palustris-Aristida stricta) ecosystems of southwestern Georgia, U.S.A. On a random basis, study sites were burned at least every other year (B), or left unburned (UB) for about 9 years. Fine root productivity and root-feeding insect abundances were sampled repeatedly across 54 random plots in UB and B sites. In Chapter 2, I characterized spatial and temporal patterns of root-feeding insect abundance, understory plant composition, soil resource availability, and fine root standing mass within each plot. Insect population densities were low overall, but abundance, patchiness, and diversity were greater in UB sites. Abundance patterns were significantly related to vegetative composition. In Chapter 3, I quantified the diet of root-feeding insects by measuring the natural abundance of carbon (C) and nitrogen (N) stable isotopes in insects and fine roots. Using 13C abundance, I examined the contribution of warm season grass roots to insect diet, relative to the proportion of warm season grass roots within adjacent root standing crop samples; 15N abundance was used to detect omnivory. Overall, insects appeared to be non-selective herbivores and omnivores that may alter foraging behavior to maintain a mixed diet (i.e. reducing or increasing warm season grass consumption when its abundance was high or low, respectively). The extent of omnivory varied within and among taxa. In Chapter 4, I estimated the top-down influence of root-feeding insects on fine root productivity by comparison of ingrowth cores with or without an insecticide treatment. I detected a weak positive effect of herbivores on the productivity of non-grass fine roots (< 10% of fine root productivity).
The ecophysiological significance of leaf movements in rhododendron-maximum
Bao, Yija; Nilsen, Erik T. (Ecological Society of America, 1988-10)
The patterns of leaf movements and their environmental stimuli have been documented for over a century. Although there have been several studies on the physiological significance of heliotropisms, thermotropic movements have been largely ignored. The objective of this study was to evaluate the ecophysiological significance of thermotropic leaf movements in a subcanopy plant through controlled experiments. Rhododendron maximum L. was selected because of its strong winter season, thermotropic leaf movements, and the ease of identifying even-aged leaf cohorts. A series of leaf manipulations was established to restrict curling, changes in leaf angle, or both. The effects of these treatments on leaf energy budget, chlorophyll content, photosynthesis, and leaf survival were evaluated. Leaves that were maintained in a horizontal position (whether they were allowed to curl or not) had the greatest decrease in chlorophyll content and the most inhibited photosynthetic rate. Reduced photosynthetic potential was due to both lower quantum yield and lower light-saturated nthetic rates. Optimal temperature for photosynthesis was several degrees above air temperature, corresponding to a horizontal leaf temperature in a sunfleck. Leaf angle influenced leaf temperature during daylight and night time; but the influence was small and most likely had no ecophysiological significance. Leaf curling had little to no influence on the ecophysiological parameters measured in this study.
Effectiveness of Treatments to Reduce Rhododendron maximum and Promote Tree Seedling Regeneration in the Southern Appalachians
Pearce, Christopher Deane (Virginia Tech, 2009-03-05)
Rosebay rhododendron (Rhododendron maximum L.) is an evergreen ericaceous shrub that plays a dynamic role in the southern Appalachian forests. Commonly located on mesic sites, this understory shrub forms dense thickets that greatly reduce the amount of light available to herbaceous and woody plants found on the forest floor. Past research has shown that silvicultural methods can be used to eradicate R. maximum, however it is unclear which of these methods is most efficient and what effects other than stem mortality may occur. In this study, treatments involving prescribed fire, mechanical cutting, and herbicide applications were applied to R. maximum dominated forests in southwestern Virginia to determine what effect seven different silvicultural treatments had on 1) controlling of R. maximum as a forest weed 2) fuel loading inside of a R. maximum thicket, and 3) canopy tree seedling regeneration. Mechanical cutting treatments were successful in reducing R. maximum basal area per acre; however stump sprouting and increased fuel loading occurred. Herbicide applications were successful in controlling only the smallest diameter class of R. maximum stems. Prescribed fire reduced litter layers and caused delayed mortality on R. maximum stems three years following treatment. Hemispherical photographs taken within each plot showed that silvicultural treatments that successfully increased the amount of light entering each plot were influential in seedling establishment three years following treatments. Results from this study can be used to further perfect silvicultural applications that alleviate R. maximum cover on the forest landscape.
The effects of forest disturbance on stream stability
Golladay, Stephen W. (Virginia Polytechnic Institute and State University, 1988)
This project was designed to examine the stability of stream ecosystems in response to forest disturbance and subsequent succession. Stability was defined as the ability of streams to retain particulate organic matter and nutrients during storms. I hypothesized that forest streams are least stable during the intermediate stages of forest succession because particulate organic matter accumulations in streams are lowest at that time. This hypothesis was tested by examining stream stability in relation to forest succession. Stream surveys indicated fewer debris dams and organic matter accumulations in streams draining early and intermediate successional forests compared to reference sites. The abundance of large wood declined within 10 years of forest disturbance and continued to decline for at least 30-40 years through the intermediate stages of forest succession. Comparisons of inputs with standing stocks of organic matter indicated that streams draining early and intermediate successional sites receive less litter from their watersheds and processed it faster. Decreases in stream obstructions combined with changes in litter inputs and processing resulted in relatively high storm transport of fine organic matter from disturbed streams. Storm organic matter export from disturbed streams averaged 4.22 g AFDW/m² and from reference streams averaged 1.83 g AFDW/m². Storm nutrient budgets, constructed by measuring nutrient inputs (soil water, throughfall) and outputs (stream discharge) during individual storms indicated that streams draining early and intermediate successional forest were less retentive of nitrogen and phosphorus than reference sites. Nitrogen loss from disturbed streams averaged 58.04 mg/m²/storm and from reference streams averaged 16.52 mg/m²/storm. Phosphorus loss from disturbed streams averaged 32.52 mg/m²/storm and from reference sites averaged 7.14 mg/m²/storm. A majority of the nitrogen and phosphorus loss was in association with organic particles. There was no difference between disturbed and reference streams in potassium, calcium, or sulfate retention during storms. However, disturbed streams tended to lose more particulate organic potassium and calcium than reference sites. These results indicate that forest disturbance has a Iong-term impact on stream ecosystems by reducing their stability for many years following forest clearing.
The effects of priming on vigor and viability of broccoli (Brassica oleracea var. italica Plenck) seeds
Jett, Lewis W. (Virginia Tech, 1994-12-15)
Seed priming is a controlled hydration process, followed by dehydration, that allows pregerminative metabolic activity to proceed without germination. The objective of this research was to investigate the effects of priming on intrinsic characteristics of seed germination including temperature, water, and development, in order to understand how priming affects the germination of broccoli (Brassica oleracea var. italica Plenck) seeds. Priming of broccoli seeds consistently improved germination and emergence rate in the laboratory and field and was related to the accumulation of a specific level of hydropriming units expressed in MPa*h. Priming reduced the sensitivity of seed germination to temperature and increased the temperature range of germination but did not lower the minimum temperature for germination. Primed seeds leaked less electrolytes at supraoptimal temperatures (≥ 35°C) compared to nonprimed seeds. In the field, primed seeds produced a greater plant stand and yield under stressful emergence conditions. Under optimal conditions in the field for stand establishment, the advancement in emergence of primed seeds did not carry over to earlier, greater yields. Matric priming, using calcium silicate as the carrier in the ratio 1.0:0.8:1.8 (seed:carrier:water; by weight) for 7 d at 20°C, was superior to osmotic priming using polyethylene glycol (pEG 8000) at -1.2 :MPa in nearly all variables examined. This may be attributed to reduced respiration during priming of seeds in PEG or nutrient uptake by seeds in calcium silicate. The testa was observed to be a barrier to broccoli seed germination. Priming-induced changes to the physical characteristics of broccoli seeds included increased volume (32%) and an irreversibly expanded, and weakened testa with some minute cracking near the area where the radicle emerges. Primed seeds germinated faster, in part by maintaining a lower hydrotime constant, and thus exhibited a greater progression towards germination per unit water potential at a constant temperature compared with nonprimed seeds. It was hypothesized that, since the testa threshold was reduced after priming by expansion and formation of free spaces, the reversibly expanded embryo of primed seeds does not become immediately appressed to the testa upon rehydration. Thus the yield threshold component controlling the rate of germination of primed seeds is lower by the amount of the testa threshold. The priming effect is more than just reducing the yield threshold as indicated by a significant invigoration of seeds with split testae. Despite the increased volume as a result of the formation of free spaces, primed broccoli seeds did not imbibe more water or have a greater turgor at full hydration. Priming did not lower the minimum water potential allowing germination, and primed seeds did not plateau in water uptake but, instead, moved immediately from imbibition to expansive growth. Priming improved the germination rate of broccoli seeds at all stages of maturity with the most significant effects at stages before attainment of maximum dry weight. Dry storage of broccoli seeds at harvest able maturity (≥ 56 days after pollination) did not improve germination, indicating a lack of post harvest dormancy.
Environmental determinism and forest structure and composition: a naturally replicated experiment
Schiffman, Paula M. (Virginia Tech, 1990)
Ecological theory states that forest succession is largely environmentally determined. Many investigators have suggested, however, that stochastic processes can frequently alter successional pathways. In particular, the colonization phase is thought to be very stochastic. This study utilizes a unique series of 6 naturally replicated sites on Brush Mountain (Montgomery Co., VA) to determine whether forest structure and species composition is primarily deterministic or stochastic in nature. Although the canopy stratum of the mature forest at these environmentally similar sites was very Similar in structure and species composition (p>0.05), the composition of the subcanopy strata vegetation differed Significantly among sites (p<0.05). This indicated that stochastic events (e.g. low intensity ground fires, deer browsing, cattle grazing) were were important factors during the development of understory and ground layer vegetation. Site-to-site differences in subcanopy species composition remained apparent during the first 2 years following disturbance (removal of canopy cover) of 4 sites. Brush Mountain lacked a significant seed bank (< 1 seed/m²), and the dominant sub canopy species reproduced largely by vegetative sprouting. Post-disturbance colonization of the sites was very stochastic, but had relatively little immediate effect on vegetation structure and composition. In the future, however, as colonist species reproduce and increase in density, stochastic colonization events may become an important aspect of forest development at some of the sites.
Evaluation of seed and seedling response to aid revegetation of hazardous chemical waste sites
Hill, Stephanie R. (Virginia Tech, 1993-05-05)
The response of several plant species to heavy metal contaminated soils was evaluated using plant bioassays with a soil substrate. A natural soil was collected from Dinwiddie County, Virginia and soil analysis was performed. The plant species, Lolium multiflorum, setaria italica and Trifolium rep ens latum, Robinia pseudoacacia, Andropogon gerardi, Asclepias syriaca, Echinacea purpurea, Rudbeckia hirta and Festuca rubra were grown in to determine the response to cupric and cadmium chloride in soils (mg Cu/kg soil). A few plant species were grown in small pots in a plant growth chamber for 28 days using control, 10, 30, 100 and 300mg Cu or Cd/kg soil. Germination proved to be less sensitive than root length. S. italica had highest ECSOs. In eu 20.7 and 15.3 in Cd. All plant species were grown for 7 days in 0.3, 1.0, 3.0 10.0, and 30.0mg Cu/kg soil and in control. Germination was not effected by metal concentrations in most species (p=0.07-0.6), except T.repens latum, R. hirta and F. rubra at 30mg/kg (p=0.0007). Root length was significantly effected by Cu concentrations for almost all species (p=0.0001-0.0112). Setaria italica had the highest EC50 at 10.86mg/kg. Robinia pseudoacacia root length was not significantly affected by CU concentrations. The other species had EC50s ranging from 3.74-7.51mg/kg. Both inhibition and stimulation of root growth were observed. Preliminary studies regarding germination rates, fungicides and rangefinding are included.
Factors affecting root system response to nutrient heterogeneity in forested wetland ecosystems
Neatrour, Matthew Aaron (Virginia Tech, 2005-04-21)
Soil nutrients are often heterogeneously distributed in space and time at scales relevant to individual plants, and plants can respond by selectively proliferating their roots within nutrient-rich patches. However, many environmental factors may increase or decrease the degree of root proliferation by plants. I explored how soil fertility, nitrogen (N) or phosphorus (P) limitation, and soil oxygen availability affected root system response to nutrient heterogeneity in forested wetland ecosystems of southeastern United States. Fine root biomass was not correlated with soil nutrient availability within wetland ecosystems, but was related to ecosystem-scale fertility. Root systems generally did not respond to P-rich patches in both floodplain (nutrient-rich) and depressional swamps (nutrient-poor) swamps, but results were inconclusive because the growth medium (sand) potentially hindered root growth. In floodplain forests, roots proliferated into N-rich patches but not P-rich patches, even though litterfall N:P ratios were > 15, which suggested that these ecosystems were P-limited. The combination of nutrient and oxygen heterogeneity affected root proliferation and biomass growth of three common floodplain forest species (Liquidambar styraciflua, Fraxinus pennsylvanica, and Nyssa aquatica) in a potted study, which was related to species' flood tolerance. My results suggest that the environmental context of plants can affect roots system response to nutrient heterogeneity in forested wetland ecosystems and highlights the need for field studies that investigate this phenomenon. Learning how environmental conditions affect plant response to nutrient heterogeneity at a fine-scale will provide better predictions of nutrient cycling, plant competition and succession, and forest productivity, which are important factors that determine carbon sequestration and timber production.

Browsing by Author "Nilsen, Erik T."

Results Per Page

Sort Options