Scholarly Works, Mechanical Engineering

Permanent URI for this collection

https://hdl.handle.net/10919/23262
Research articles, presentations, and other scholarship

Browse

Browsing Scholarly Works, Mechanical Engineering by Department "Chemical Engineering"

Now showing 1 - 6 of 6
  • Thumbnail Image
    Correlations between the thermal vibrations of two cantilevers: Validation of deterministic analysis via the fluctuation-dissipation theorem
    Honig, Christopher D. F.; Radiom, Milad; Robbins, Brian A.; Walz, John Y.; Paul, Mark R.; Ducker, William A. (AIP Publishing, 2012-02-01)
    We validate a theoretical approach for analyzing correlations in the fluctuations of two cantilevers in terms of a deterministic model, using the fluctuation-dissipation theorem [M. R. Paul and M. C. Cross, Phys. Rev. Lett. 92, 235501 (2004)]. The validation has been made possible through measurement of the correlations between the thermally stimulated vibrations of two closely spaced micrometer-scale cantilevers in fluid. Validation of the theory enables development of a method for characterizing fluids, which we call correlation force spectrometry. (C) 2012 American Institute of Physics. [doi:10.1063/1.3681141]
  • Thumbnail Image
    The influence of interface bonding on thermal transport through solid-liquid interfaces
    Harikrishna, H.; Ducker, William A.; Huxtable, Scott T. (AIP Publishing, 2013-06-01)
    We use time-domain thermoreflectance to show that interface thermal conductance, G, is proportional to the thermodynamic work of adhesion between gold and water, W-SL, for a series of five alkane-thiol monolayers at the gold-water interface. W-SL is a measure of the bond strength across the solid-liquid interface. Differences in bond strength, and thus differences in W-SL, are achieved by varying the terminal group (omega-group) of the alkane-thiol monolayers on the gold. The interface thermal conductance values were in the range 60-190 MW m(-2) K-1, and the solid-liquid contact angles span from 25 degrees to 118 degrees. (C) 2013 AIP Publishing LLC.
  • Thumbnail Image
    Modeling iontophoretic drug delivery in a microfluidic device
    Moarefian, Maryam; Davalos, Rafael V.; Tafti, Danesh K.; Achenie, Luke E. K.; Jones, Caroline N. (2020-09-21)
    Iontophoresis employs low-intensity electrical voltage and continuous constant current to direct a charged drug into a tissue. Iontophoretic drug delivery has recently been used as a novel method for cancer treatment in vivo. There is an urgent need to precisely model the low-intensity electric fields in cell culture systems to optimize iontophoretic drug delivery to tumors. Here, we present an iontophoresis-on-chip (IOC) platform to precisely quantify carboplatin drug delivery and its corresponding anti-cancer efficacy under various voltages and currents. In this study, we use an in vitro heparin-based hydrogel microfluidic device to model the movement of a charged drug across an extracellular matrix (ECM) and in MDA-MB231 triple-negative breast cancer (TNBC) cells. Transport of the drug through the hydrogel was modeled based on diffusion and electrophoresis of charged drug molecules in the direction of an oppositely charged electrode. The drug concentration in the tumor extracellular matrix was computed using finite element modeling of transient drug transport in the heparin-based hydrogel. The model predictions were then validated using the IOC platform by comparing the predicted concentration of a fluorescent cationic dye (Alexa Fluor 594 (R)) to the actual concentration in the microfluidic device. Alexa Fluor 594 (R) was used because it has a molecular weight close to paclitaxel, the gold standard drug for treating TNBC, and carboplatin. Our results demonstrated that a 50 mV DC electric field and a 3 mA electrical current significantly increased drug delivery and tumor cell death by 48.12% +/- 14.33 and 39.13% +/- 12.86, respectively (n = 3, p-value <0.05). The IOC platform and mathematical drug delivery model of iontophoresis are promising tools for precise delivery of chemotherapeutic drugs into solid tumors. Further improvements to the IOC platform can be made by adding a layer of epidermal cells to model the skin.
  • Thumbnail Image
    Nanoscale Bacteria‐Enabled Autonomous Drug Delivery System (NanoBEADS) Enhances Intratumoral Transport of Nanomedicine
    Suh, SeungBeum; Jo, Ami; Traore, Mahama Aziz; Zhan, Ying; Coutermarsh-Ott, Sheryl; Ringel-Scaia, Veronica M.; Allen, Irving C.; Davis, Richey M.; Behkam, Bahareh (Wiley, 2018-12-05)
    Cancer drug delivery remains a formidable challenge due to systemic toxicity and inadequate extravascular transport of nanotherapeutics to cells distal from blood vessels. It is hypothesized that, in absence of an external driving force, the Salmonella enterica serovar Typhimurium could be exploited for autonomous targeted delivery of nanotherapeutics to currently unreachable sites. To test the hypothesis, a nanoscale bacteria‐enabled autonomous drug delivery system (NanoBEADS) is developed in which the functional capabilities of the tumor‐targeting S. Typhimurium VNP20009 are interfaced with poly(lactic‐co‐glycolic acid) nanoparticles. The impact of nanoparticle conjugation is evaluated on NanoBEADS' invasion of cancer cells and intratumoral transport in 3D tumor spheroids in vitro, and biodistribution in a mammary tumor model in vivo. It is found that intercellular (between cells) self‐replication and translocation are the dominant mechanisms of bacteria intratumoral penetration and that nanoparticle conjugation does not impede bacteria's intratumoral transport performance. Through the development of new transport metrics, it is demonstrated that NanoBEADS enhance nanoparticle retention and distribution in solid tumors by up to a remarkable 100‐fold without requiring any externally applied driving force or control input. Such autonomous biohybrid systems could unlock a powerful new paradigm in cancer treatment by improving the therapeutic index of chemotherapeutic drugs and minimizing systemic side effects.
  • Thumbnail Image
    A pressure gauge based on gas density measurement from analysis of the thermal noise of an atomic force microscope cantilever
    Seo, Dongjin; Paul, Mark R.; Ducker, William A. (AIP Publishing, 2012-05-01)
    We describe a gas-density gauge based on the analysis of the thermally-driven fluctuations of an atomic force microscope (AFM) cantilever. The fluctuations are modeled as a ring-down of a simple harmonic oscillator, which allows fitting of the resonance frequency and damping of the cantilever, which in turn yields the gas density. The pressure is obtained from the density using the known equation of state. In the range 10-220 kPa, the pressure readings from the cantilever gauge deviate by an average of only about 5% from pressure readings on a commercial gauge. The theoretical description we use to determine the pressure from the cantilever motion is based upon the continuum hypothesis, which sets a minimum pressure for our analysis. It is anticipated that the cantilever gauge could be extended to measure lower pressures given a molecular theoretical description. Alternatively, the gauge could be calibrated for use in the non-continuum range. Our measurement technique is similar to previous AFM cantilever measurements, but the analysis produces improved accuracy. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4717678]
  • Thumbnail Image
    Rheology of fluids measured by correlation force spectroscopy
    Radiom, Milad; Robbins, Brian A.; Honig, Christopher D. F.; Walz, John Y.; Paul, Mark R.; Ducker, William A. (AIP Publishing, 2012-04-01)
    We describe a method, correlation force spectrometry (CFS), which characterizes fluids through measurement of the correlations between the thermally stimulated vibrations of two closely spaced micrometer-scale cantilevers in fluid. We discuss a major application: measurement of the rheological properties of fluids at high frequency and high spatial resolution. Use of CFS as a rheometer is validated by comparison between experimental data and finite element modeling of the deterministic ring-down of cantilevers using the known viscosity of fluids. The data can also be accurately fitted using a harmonic oscillator model, which can be used for rapid rheometric measurements after calibration. The method is non-invasive, uses a very small amount of fluid, and has no actively moving parts. It can also be used to analyze the rheology of complex fluids. We use CFS to show that (non-Newtonian) aqueous polyethylene oxide solution can be modeled approximately by incorporating an elastic spring between the cantilevers. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4704085]