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dc.contributor.authorHe, Xinen
dc.date.accessioned2021-04-07T06:00:17Zen
dc.date.available2021-04-07T06:00:17Zen
dc.date.issued2019-10-14en
dc.identifier.othervt_gsexam:22673en
dc.identifier.urihttp://hdl.handle.net/10919/102956en
dc.description.abstractLysM-domain containing (LysMD) proteins are widespread in nature and associated with host-pathogen interactions, often-binding peptidoglycan and chitin. However, the functions of mammalian LysMD proteins have not been fully defined. Chitin, a major component of fungal cell walls, has been associated with allergic disorders such as asthma. However, chitin recognition by mammals remains enigmatic at best. The principal receptor(s) on epithelial cells for chitin recognition remain to be determined. In this study, we demonstrate that LYSMD3 is expressed on the surface of human airway epithelial cells. Interestingly, LYSMD3 is able to bind chitin and β-glucan as well as fungal spores. Knockdown and knockout of LYSMD3 markedly impaired chitin and fungi-induced inflammatory cytokine production in lung epithelial cells. Antagonization of LYSMD3 ectodomain by soluble LYSMD3 protein, multiple ligands, or antibody against LYSMD3 all significantly blocked chitin signaling. Taken together our study identifies LYSMD3 as a mammalian pattern recognition receptor (PRR) for chitin and is required for the epithelial inflammatory response to chitin and fungal spores.en
dc.format.mediumETDen
dc.publisherVirginia Techen
dc.rightsThis item is protected by copyright and/or related rights. Some uses of this item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en
dc.subjectallergyen
dc.subjectAlternariaen
dc.subjectasthmaen
dc.subjectchitinen
dc.subjectinnate immunityen
dc.subjectLYSMD3en
dc.subjectpattern recognition receptoren
dc.titleIdentification and Characterization of LYSMD3, A Novel Epithelial Cell Pattern Recognition Receptor for Chitinen
dc.typeDissertationen
dc.contributor.departmentBiological Sciencesen
dc.description.degreeDoctor of Philosophyen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.leveldoctoralen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineBiological Sciencesen
dc.contributor.committeechairLawrence, Christopher B.en
dc.contributor.committeememberJones, Caroline N.en
dc.contributor.committeememberBuechner-Maxwell, Virginia A.en
dc.contributor.committeememberLi, Liwuen


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