• Log in
    View Item 
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Doctoral Dissertations
    • View Item
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Prohexadione Calcium for Turfgrass Management and Poa annua Control and Molecular Assessment of the Acetolactate Synthase Gene in Poa annua

    Thumbnail
    View/Open
    etd.pdf (561.3Kb)
    Downloads: 255
    Date
    2004-04-27
    Author
    Beam, Joshua Bart
    Metadata
    Show full item record
    Abstract
    Managing turf for high aesthetic value is costly. Such management usually involves mowing, disease prevention, insect control, and weed control. Mowing is the most expensive practice on golf courses and annual bluegrass (Poa annua L) is the most challenging weed problem in professional turf. The plant growth regulators trinexapac-ethyl and paclobutrazol are commonly used in VA for these two costly and challenging jobs. Prohexadione calcium (PC) is an experimental chemical that inhibits the same enzyme (3ß-hydroxyalase) as trinexapac-ethyl and may selectively suppress annual bluegrass. Experiments were conducted at the Virginia Tech Turfgrass Research Center and Glade Road Research Facility to determine the PC rate required to reduce clipping biomass of four turfgrass species as effectively as trinexapac-ethyl. Prohexadione calcium reduced clipping biomass of bermudagrass (Cynodon dactylon (L.) Pers.), Kentucky bluegrass (Poa pratenis L.), perennial ryegrass (Lolium perenne L.), and zoysiagrass (Zoysia japonica Steud.) equivalent to trinexapac-ethyl at 0.70, 0.22, 0.60, and 0.27 kg a.i./ha -1, respectively. Further experiments conducted at three locations across Virginia determined that PC was comparable to paclobutrazol for annual bluegrass suppression. Since turfgrass response to PC was different between annual bluegrass, Kentucky bluegrass, and perennial ryegrass, 14C labeled PC was used to assess absorption, translocation, and metabolism of PC between annual and Kentucky bluegrass, creeping bentgrass (Agrostis stolonifera L.), and perennial ryegrass. Annual and Kentucky bluegrass absorbed more PC than creeping bentgrass or perennial ryegrass and partially explained the selectivity between these species. Translocation and metabolism of PC did not differ between species. Our final objective launched experiments characterizing possible resistance to acetolactate synthase (ALS) inhibiting herbicides in annual bluegrass. Several selective herbicides for annual bluegrass control inhibit ALS. Since many weeds have developed resistance to ALS-inhibiting herbicides, the ALS gene in annual bluegrass was sequenced and derived amino acid sequences were at least 87% similar to other previously sequenced grass species. This sequencing data will be used in future experiments to predict the likelihood of ALS resistance in annual bluegrass.
    URI
    http://hdl.handle.net/10919/11176
    Collections
    • Doctoral Dissertations [13032]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us