The internal vertebral venous plexus (IVVP) is an extensive vascular network recently implicated in various human and canine spinal disorders. Nevertheless, little recent information is available regarding normal anatomy of canine IVVP and its role in acute spinal injuries. The objectives of the study were; (1) to describe the normal IVVP morphology in the canine cervical region using transverse anatomy sections and computed tomography (CT), (2) to develop a technique for CT examination of the IVVP in vivo, (3) to analyze the quantitative characteristics of the IVVP, and (4) to assess the effect of acute experimental spinal cord compression on IVVP morphology. In the first experiment, CT of the cervical vertebral canal was performed in 6, normal, adult mixed-breed dogs. After dogs were euthanized, a gelatin and iothalamate mixture was injected into the right external jugular vein. Cadavers were then frozen to â 8°C, sliced into transverse sections, and compared with CT images. Vascular components such as the IVVP, interarcuate veins, intervertebral veins, and vertebral veins were accurately depicted on CT images. In the second experiment, CT venography was performed using a biphasic IV injection of iodinated contrast medium. Dimensions of the IVVP and other vertebral canal components were calculated for the C3-C7 vertebral region. Sagittal diameters of the IVVP ranged from 0.6 mm to 3.2 mm. The IVVP area occupied 30.61% of the cervical vertebral epidural space area. When C3-C7 segments were considered as a group, IVVP area dimensions were significantly correlated (r > 0.7, p < 0.0001) with vertebral canal area and dural sac area. In the last experiment, acute spinal cord compression (ASCC) was induced and maintained for 10 minutes using an angioplasty balloon catheter device over the C3/4 vertebral region in 6 dogs. Dogs were evaluated prior to, during, and after compression using digital subtraction venography (DSV) and CT venography. Results showed that ASCC produced a significant change in diameter of the IVVP at the site of compression. This effect persisted during the post-compression period. In conclusion, findings indicate that CT venography and DSV accurately depict the IVVP in dogs, and that significant changes of the IVVP morphology occur under ASCC conditions.