GROWTH ANALYSES AND PATTERNS OF CROSS-RESISTANCE IN FOUR IMIDAZOLINONE-RESISTANT SMOOTH PIGWEED (Amaranthus hybridus) POPULATIONS
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Absorption, translocation, and metabolism of 14C-cloransulam-methyl in S and R2 populations were generally similar. Three metabolites of cloransulam-methyl with ratio of front (Rf) values approximately 0.83, 0.65, and 0.45 were isolated. The metabolite with a 0.83 Rf value increased over time as the parent molecule decreased indicating that it plays a major role in cloransulam-methyl metabolism in smooth pigweed. The other metabolites did not change significantly over time and never represented more than 5 percent of the extracted radioactivity. The identity of these metabolites has not been determined.
Using enzyme assays, it was determined that IMI resistance in R populations was due to an altered ALS that was no longer susceptible to inhibition by these herbicides. ALS from S, R1, and R2 populations responded similarly to chlorimuron and thifensulfuron, but reductions in enzyme activity by chlorimuron and thifensulfuron were significantly greater for R3 ALS than for S, R1 or R2 ALS. ALS from R2 and R3 was significantly more sensitive to inhibition by pyrithiobac compared to S ALS. Based on resistance ratios, R2 and R3 ALS were also more sensitive to inhibition by cloransulam-methyl than S ALS. Negative cross-resistance to thifensulfuron, pyrithiobac, and cloransulam-methyl in some R populations at the whole-plant level can be explained by increased sensitivity at the enzyme level.
Under noncompetitive conditions in the greenhouse, S produced 17, 23, 25, and 44 percent more biomass than R1, R2, R3, and R4 populations, respectively. S plants were also taller than R plants 17 and 21 d after planting (DAP) and displayed a faster initial rate of leaf area increase compared to all R populations. The net assimilation rate of S was significantly higher than R2 and R3 populations 24 DAP. R3 and R4 populations had significantly less chlorophyll per g of plant tissue compared to S; therefore, reduced growth in some R populations compared to S may be linked to chlorosis that generally appears early in seedling development. Biomass production in the field under competitive conditions was similar for all populations using both monoculture and mixed populations. For this reason, the differences in growth observed in the greenhouse in the S population may not confer a competitive advantage over R populations in the field.
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