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dc.contributor.authorLi, Shenghuaen_US
dc.date.accessioned2014-03-14T20:17:54Z
dc.date.available2014-03-14T20:17:54Z
dc.date.issued2008-10-24en_US
dc.identifier.otheretd-11032008-113406en_US
dc.identifier.urihttp://hdl.handle.net/10919/29442
dc.description.abstractCaulobacter crescentus is an important model organism for studying regulation of cell growth, division and differentiation in prokaryotes. C. crescentus undergoes asymmetric division producing two progeny cells with identical genome but different developmental programs: the "swarmer" cell is flagellated and motile, and the "stalked" cell is sessile (attached to a surface by its stalk and holdfast). Only stalked cells undergo chromosome replication and cell division. A swarmer cell must shed its flagellum and grow a stalk before it can enter the replication-division cycle. Based on published experimental evidence, we propose a molecular mechanism controlling the cell division cycle in this bacterium. Our quantitative model of the mechanism illustrates detailed temporal dynamics of regulatory proteins and corresponding physiological changes during the process of cell cycle progression and differentiation of wild-type cells (both stalked cells and swarmer cells) and of a number of known and novel mutant strains. Our model presents a unified view of temporal regulation of protein activities during the asymmetric cell division cycle of C. crescentus and provides an opportunity to study and analyze the system dynamics of the Caulobacter cell cycle (as opposed to the dynamics of individual steps). The model can serve as a starting point for investigating molecular regulations of cell division and differentiation in other genera of alpha-proteobacteria, such as Brucella and Rhizobium, because recent experimental data suggest that these alpha-proteobacteria share similar genetic mechanisms for cell cycle control.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartSL_Dissertation.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectprotein networken_US
dc.subjectcell cycleen_US
dc.subjectMathematical modelingen_US
dc.subjectCaulobacter crescentusen_US
dc.titleQuantitative Modeling of the Molecular Mechanism Controlling the Asymmetric Cell Division Cycle in Caulobacter crescentusen_US
dc.typeDissertationen_US
dc.contributor.departmentGenetics, Bioinformatics, and Computational Biologyen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineGenetics, Bioinformatics, and Computational Biologyen_US
dc.contributor.committeechairTyson, John J.en_US
dc.contributor.committeememberBrazhnik, Paulen_US
dc.contributor.committeememberSobral, Brunoen_US
dc.contributor.committeememberYang, Zhaominen_US
dc.contributor.committeememberLaubenbacher, Reinhard C.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11032008-113406/en_US
dc.date.sdate2008-11-03en_US
dc.date.rdate2008-12-11
dc.date.adate2008-12-11en_US


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