Investigation of mass spectrometric interfaces for supercritical fluid chromatography and liquid chromatography
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Packed column SFC, however, is only able to handle directly non-polar to medium polar analytes. Thus for polar to highly polar analytes (peptides, proteins) liquid introduction (infusion, flow injection, chromatography) is the preferred method of sample delivery to the MS. Furthermore because of the polar nature and thermal lability of these compounds conventional ionization methods (EI, CI) are not suitable. Factors which have ramification on sample handling (flow rate, solution composition) were studied. The high sample flow rate capability was dependent on effective nebulization and desolvation. Thus, needle distance/angle and bath gas flow setting played a critical role in the performance of the ES-MS. The utility of the system was demonstrated by analysis of gramicidin s, myoglobin, and tryptic peptides of cytochrome c.
- Doctoral Dissertations