Characterization of the glpD and glpEGR operons of Escherichia coli k-12
MetadataShow full item record
The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the glp regulon of Escherichia coli and are under negative transcriptional regulation by the glpR-encoded repressor. The glpR gene is adjacent to, and is transcribed divergently from, glpD. GlpR and glpD are separated by two open reading frames, designated glpE and glpG, encoding proteins of unknown function. The glpD-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of glpD was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a glpD-lacZ- translational fusion.
- Doctoral Dissertations